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An optimized microRNA backbone for effective single-copy RNAi.
Fellmann C, Hoffmann T, Sridhar V, Hopfgartner B, Muhar M, Roth M, Lai DY, Barbosa IA, Kwon JS, Guan Y, Sinha N, Zuber J
Cell Rep. 2013 Dec 26;5(6):1704-13. doi: 10.1016/j.celrep.2013.11.020. Epub 2013 Dec 12.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
111160LEPGmiR-E (miR-30 variant)-based RNAi with GFP reporter
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111161LEPZmiR-E (miR-30 variant)-based RNAi with ZsGreen reporter
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111162LENGmiR-E (miR-30 variant)-based RNAi with GFP reporter
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111163LENCmiR-E (miR-30 variant)-based RNAi with mCherry reporter
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111164RT3GEPTet-regulated miR-E (miR-30 variant)-based RNAi with tGFP reporter
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111165RT3GENTet-regulated miR-E (miR-30 variant)-based RNAi with tGFP reporter
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111166RT3RENTet-regulated miR-E (miR-30 variant)-based RNAi with DsRed reporter
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111167RT3REVINTet-regulated miR-E (miR-30 variant)-based RNAi
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111168RT3REVIRTet-regulated miR-E (miR-30 variant)-based RNAi
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111169RT3GEPIRTet-regulated miR-E (miR-30 variant)-based RNAi
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111170SGEPmiR-E (miR-30 variant)-based RNAi
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111171SGENmiR-E (miR-30 variant)-based RNAi
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111172LT3GEPTet-regulated miR-E (miR-30 variant)-based RNAi
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111173LT3GENTet-regulated miR-E (miR-30 variant)-based RNAi
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111174LT3REVTet-regulated miR-E (miR-30 variant)-based RNAi
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111175LT3REVINTet-regulated miR-E (miR-30 variant)-based RNAi
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111176LT3REVIRTet-ON miR-E (miR-30 variant)-based RNAi
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111177LT3GEPIRTet-ON miR-E (miR-30 variant)-based RNAi
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111178LT3GECIRTet-ON miR-E (miR-30 variant)-based RNAi
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