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Lentiviral transduction of mammalian cells for fast, scalable and high-level production of soluble and membrane proteins.
Elegheert J, Behiels E, Bishop B, Scott S, Woolley RE, Griffiths SC, Byrne EFX, Chang VT, Stuart DI, Jones EY, Siebold C, Aricescu AR
Nat Protoc. 2018 Nov 19. pii: 10.1038/s41596-018-0075-9. doi: 10.1038/s41596-018-0075-9.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
113883pHR-CMV-TetO2_3C-Twin-StrepMammalian (inducible) protein expression
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113884pHR-CMV-TetO2_3C-Twin-Strep_IRES-EmGFPMammalian (inducible) protein expression, bicistronic expression of cytosolic EmGFP
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113885pHR-CMV-TetO2_3C-Twin-Strep_IRES-mRuby2Mammalian (inducible) protein expression, bicistronic expression of cytosolic mRuby2
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113886pHR-CMV-TetO2_3C-Twin-Strep_IRES-mTurquoise2Mammalian (inducible) protein expression, bicistronic expression of cytosolic mTurquoise2
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113887pHR-CMV-TetO2_3C-Avi-His6Mammalian (inducible) protein expression, in vivo biotinylation
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113888pHR-CMV-TetO2_3C-Avi-His6__IRES-EmGFPMammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic EmGFP
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113889pHR-CMV-TetO2_3C-Avi-His6_IRES-mRuby2Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mRuby2
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113890pHR-CMV-TetO2_3C-Avi-His6_IRES-mTurquoise2Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mTurquoise2
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113891pHR-CMV-TetO2_3C-mVenus-Twin-StrepMammalian (inducible) protein expression, C-terminal 3C-mVenus-Twin-Strep fusion
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113892pHR-CMV-TetO2_EmGFPMammalian (inducible) expression of cytosolic EmGFP
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113893pHR-CMV-TetO2_mVenusMammalian (inducible) expression of cytosolic mVenus
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113894pHR-CMV-TetO2_mRuby2Mammalian (inducible) expression of cytosolic mRuby2
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113895pHR-CMV-TetO2_mTurquoise2Mammalian (inducible) expression of cytosolic mTurquoise2
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113896pHR-CMV-TetO2_HA-BirAMammalian (inducible) expression of HA-tagged cytosolic E. coli biotin ligase
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113897pHR-CMV-TetO2_HA-BirA-ERMammalian (inducible) expression of HA-tagged ER-resident E. coli biotin ligase
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113898pHR-CMV-TetO2_3C-Avi-His6_IRES-HA-BirA-ERMammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of HA-tagged ER-resident E. coli biotin ligase
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113899pHR-CMV_TetR-HA-NLS-P2A-BSD-MycMammalian constitutive expression of HA-tagged Tetracyclin repressor protein and Myc-tagged blasticidin deaminase
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113900pHR-SFFV_3C-Twin-StrepMammalian protein expression
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113901pHR-CAG_3C-Twin-StrepMammalian protein expression
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