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pHR-CMV-TetO2_3C-Twin-Strep_IRES-mTurquoise2
(Plasmid #113886)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 113886 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHR-CMV-TetO2
  • Vector type
    Lentiviral
  • Selectable markers
    mTurquoise2
  • Tag / Fusion Protein
    • 3C-Twin-Strep (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Promoter CMV-MIE-TetO2
  • Tag / Fusion Protein
    • 3C-Twin-Strep (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI, AgeI (not destroyed)
  • 3′ cloning site KpnI, XhoI (not destroyed)
  • 5′ sequencing primer CGTCGTCGTGCTCGTTTAGTG
  • 3′ sequencing primer CAAACGCACACCGGCCTTATTCCAAG
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This empty backbone contains a 12bp dummy insert.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHR-CMV-TetO2_3C-Twin-Strep_IRES-mTurquoise2 was a gift from A. Radu Aricescu (Addgene plasmid # 113886 ; http://n2t.net/addgene:113886 ; RRID:Addgene_113886)
  • For your References section:

    Lentiviral transduction of mammalian cells for fast, scalable and high-level production of soluble and membrane proteins. Elegheert J, Behiels E, Bishop B, Scott S, Woolley RE, Griffiths SC, Byrne EFX, Chang VT, Stuart DI, Jones EY, Siebold C, Aricescu AR. Nat Protoc. 2018 Nov 19. pii: 10.1038/s41596-018-0075-9. doi: 10.1038/s41596-018-0075-9. 10.1038/s41596-018-0075-9 PubMed 30455477