Jorge Ferrer Lab:
Ferrer Lab Plasmids
Unpublished
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 118154 | Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR | Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences. |
| 118156 | Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP | Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences. |
| 118157 | Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR BFP-guide1 | CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the BFP CDS |
| 118158 | Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide1 | CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the EGFP CDS |
| 118159 | Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide2 | CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA2 against the EGFP CDS |
| 118160 | Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide3 | CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA3 against the EGFP CDS |