PurposeCatalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||118156||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerFeng Zhang (Addgene #52961)
Vector typeMammalian Expression, Lentiviral, CRISPR
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)4907
- Promoter EF1a core and U6
/ Fusion Protein
- HA (C terminal on insert)
- Cloning method Gibson Cloning
- 5′ sequencing primer TAAGAAGTACGGCGGCTTCG (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Note: Plasmid contains a N1396S mutation in dCas9. This mutation is not known to affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP was a gift from Jorge Ferrer (Addgene plasmid # 118156 ; http://n2t.net/addgene:118156 ; RRID:Addgene_118156)