Mapping Cellular Reprogramming via Pooled Overexpression Screens with Paired Fitness and Single-Cell RNA-Sequencing Readout.
Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 10.1016/j.cels.2018.10.008. PubMed Article
Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 10.1016/j.cels.2018.10.008. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 120426 | EF1a_mCherry_P2A_Hygro_Barcode | Barcoded lentiviral vector to express mCherry in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120427 | EF1a_ASCL1_P2A_Hygro_Barcode | Barcoded lentiviral vector to express ASCL1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120428 | EF1a_ASCL3_P2A_Hygro_Barcode | Barcoded lentiviral vector to express ASCL3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120429 | EF1a_ASCL4_P2A_Hygro_Barcode | Barcoded lentiviral vector to express ASCL4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120430 | EF1a_ASCL5_P2A_Hygro_Barcode | Barcoded lentiviral vector to express ASCL5 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120432 | EF1a_CDX2_P2A_Hygro_Barcode | Barcoded lentiviral vector to express CDX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120433 | EF1a_CRX_P2A_Hygro_Barcode | Barcoded lentiviral vector to express CRX in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120434 | EF1a_ERG_P2A_Hygro_Barcode | Barcoded lentiviral vector to express ERG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120435 | EF1a_ESRRG_P2A_Hygro_Barcode | Barcoded lentiviral vector to express ESRRG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120436 | EF1a_ETV2_P2A_Hygro_Barcode | Barcoded lentiviral vector to express ETV2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120437 | EF1a_FLI1_P2A_Hygro_Barcode | Barcoded lentiviral vector to express FLI1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120438 | EF1a_FOXA1_P2A_Hygro_Barcode | Barcoded lentiviral vector to express FOXA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120439 | EF1a_FOXA2_P2A_Hygro_Barcode | Barcoded lentiviral vector to express FOXA2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120440 | EF1a_FOXA3_P2A_Hygro_Barcode | Barcoded lentiviral vector to express FOXA3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120441 | EF1a_FOXP1_P2A_Hygro_Barcode | Barcoded lentiviral vector to express FOXP1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120442 | EF1a_GATA1_P2A_Hygro_Barcode | Barcoded lentiviral vector to express GATA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120444 | EF1a_GATA4_P2A_Hygro_Barcode | Barcoded lentiviral vector to express GATA4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120445 | EF1a_GATA6_P2A_Hygro_Barcode | Barcoded lentiviral vector to express GATA6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120447 | EF1a_HAND2_P2A_Hygro_Barcode | Barcoded lentiviral vector to express HAND2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120448 | EF1a_HNF1A_P2A_Hygro_Barcode | Barcoded lentiviral vector to express HNF1A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120450 | EF1a_HNF4A_P2A_Hygro_Barcode | Barcoded lentiviral vector to express HNF4A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120451 | EF1a_HOXA1_P2A_Hygro_Barcode | Barcoded lentiviral vector to express HOXA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120452 | EF1a_HOXA10_P2A_Hygro_Barcode | Barcoded lentiviral vector to express HOXA10 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120453 | EF1a_HOXA11_P2A_Hygro_Barcode | Barcoded lentiviral vector to express HOXA11 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120454 | EF1a_HOXB6_P2A_Hygro_Barcode | Barcoded lentiviral vector to express HOXB6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120455 | EF1a_KLF4_P2A_Hygro_Barcode | Barcoded lentiviral vector to express KLF4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120456 | EF1a_LHX3_P2A_Hygro_Barcode | Barcoded lentiviral vector to express LHX3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120457 | EF1a_LMX1A_P2A_Hygro_Barcode | Barcoded lentiviral vector to express LMX1A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120458 | EF1a_MEF2C_P2A_Hygro_Barcode | Barcoded lentiviral vector to express MEF2C in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120459 | EF1a_MESP1_P2A_Hygro_Barcode | Barcoded lentiviral vector to express MESP1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120460 | EF1a_MITF_P2A_Hygro_Barcode | Barcoded lentiviral vector to express MITF in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120461 | EF1a_MYC_P2A_Hygro_Barcode | Barcoded lentiviral vector to express MYC in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120462 | EF1a_MYCL_P2A_Hygro_Barcode | Barcoded lentiviral vector to express MYCL in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120463 | EF1a_MYCN_P2A_Hygro_Barcode | Barcoded lentiviral vector to express MYCN in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120464 | EF1a_MYOD1_P2A_Hygro_Barcode | Barcoded lentiviral vector to express MYOD1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120465 | EF1a_MYOG_P2A_Hygro_Barcode | Barcoded lentiviral vector to express MYOG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120466 | EF1a_NEUROD1_P2A_Hygro_Barcode | Barcoded lentiviral vector to express NEUROD1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120467 | EF1a_NEUROG1_P2A_Hygro_Barcode | Barcoded lentiviral vector to express NEUROG1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120468 | EF1a_NEUROG3_P2A_Hygro_Barcode | Barcoded lentiviral vector to express NEUROG3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120469 | EF1a_NRL_P2A_Hygro_Barcode | Barcoded lentiviral vector to express NRL in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120470 | EF1a_ONECUT1_P2A_Hygro_Barcode | Barcoded lentiviral vector to express ONECUT1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120471 | EF1a_OTX2_P2A_Hygro_Barcode | Barcoded lentiviral vector to express OTX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120472 | EF1a_PAX7_P2A_Hygro_Barcode | Barcoded lentiviral vector to express PAX7 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120473 | EF1a_POU1F1_P2A_Hygro_Barcode | Barcoded lentiviral vector to express POU1F1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120474 | EF1a_POU5F1_P2A_Hygro_Barcode | Barcoded lentiviral vector to express POU5F1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120475 | EF1a_RUNX1_P2A_Hygro_Barcode | Barcoded lentiviral vector to express RUNX1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120476 | EF1a_SIX1_P2A_Hygro_Barcode | Barcoded lentiviral vector to express SIX1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120477 | EF1a_SIX2_P2A_Hygro_Barcode | Barcoded lentiviral vector to express SIX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120478 | EF1a_SNAI2_P2A_Hygro_Barcode | Barcoded lentiviral vector to express SNAI2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120479 | EF1a_SOX10_P2A_Hygro_Barcode | Barcoded lentiviral vector to express SOX10 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120480 | EF1a_SOX2_P2A_Hygro_Barcode | Barcoded lentiviral vector to express SOX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120481 | EF1a_SOX3_P2A_Hygro_Barcode | Barcoded lentiviral vector to express SOX3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120482 | EF1a_SPI1_P2A_Hygro_Barcode | Barcoded lentiviral vector to express SPI1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120483 | EF1a_SPIB_P2A_Hygro_Barcode | Barcoded lentiviral vector to express SPIB in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120484 | EF1a_SPIC_P2A_Hygro_Barcode | Barcoded lentiviral vector to express SPIC in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120485 | EF1a_SRY_P2A_Hygro_Barcode | Barcoded lentiviral vector to express SRY in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120486 | EF1a_TBX5_P2A_Hygro_Barcode | Barcoded lentiviral vector to express TBX5 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120487 | EF1a_TFAP2C_P2A_Hygro_Barcode | Barcoded lentiviral vector to express TFAP2C in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120488 | EF1a_KLF1_P2A_Hygro_Barcode | Barcoded lentiviral vector to express KLF1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120489 | EF1a_KLF2_P2A_Hygro_Barcode | Barcoded lentiviral vector to express KLF2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120490 | EF1a_KLF3_P2A_Hygro_Barcode | Barcoded lentiviral vector to express KLF3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120491 | EF1a_KLF5_P2A_Hygro_Barcode | Barcoded lentiviral vector to express KLF5 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120492 | EF1a_KLF6_P2A_Hygro_Barcode | Barcoded lentiviral vector to express KLF6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120493 | EF1a_KLF7_P2A_Hygro_Barcode | Barcoded lentiviral vector to express KLF7 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120494 | EF1a_KLF8_P2A_Hygro_Barcode | Barcoded lentiviral vector to express KLF8 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120495 | EF1a_KLF9_P2A_Hygro_Barcode | Barcoded lentiviral vector to express KLF9 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120496 | EF1a_KLF10_P2A_Hygro_Barcode | Barcoded lentiviral vector to express KLF10 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120497 | EF1a_KLF11_P2A_Hygro_Barcode | Barcoded lentiviral vector to express KLF11 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120498 | EF1a_KLF12_P2A_Hygro_Barcode | Barcoded lentiviral vector to express KLF12 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120499 | EF1a_KLF13_P2A_Hygro_Barcode | Barcoded lentiviral vector to express KLF13 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120500 | EF1a_KLF14_P2A_Hygro_Barcode | Barcoded lentiviral vector to express KLF14 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120501 | EF1a_KLF15_P2A_Hygro_Barcode | Barcoded lentiviral vector to express KLF15 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120502 | EF1a_KLF16_P2A_Hygro_Barcode | Barcoded lentiviral vector to express KLF16 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120503 | EF1a_KLF17_P2A_Hygro_Barcode | Barcoded lentiviral vector to express KLF17 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120504 | EF1a_MYC deltaMBI_P2A_Hygro_Barcode | Barcoded lentiviral vector to express MYC deltaMBI in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120505 | EF1a_MYC deltaMBII_P2A_Hygro_Barcode | Barcoded lentiviral vector to express c-MYC deltaMBII in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120506 | EF1a_MYC deltaNLS_P2A_Hygro_Barcode | Barcoded lentiviral vector to express c-MYC deltaNLS in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120507 | EF1a_MYC deltab_P2A_Hygro_Barcode | Barcoded lentiviral vector to express MYC deltab in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120508 | EF1a_MYC deltaHLH_P2A_Hygro_Barcode | Barcoded lentiviral vector to express MYC deltaHLH in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120509 | EF1a_MYC deltaLZ_P2A_Hygro_Barcode | Barcoded lentiviral vector to express MYC deltaLZ in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120510 | EF1a_MYC deltaNTD_P2A_Hygro_Barcode | Barcoded lentiviral vector to express MYC deltaNTD in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120511 | EF1a_MYC deltaCTD_P2A_Hygro_Barcode | Barcoded lentiviral vector to express MYC deltaCTD in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120512 | EF1a_MYC Glu39Ala_P2A_Hygro_Barcode | Barcoded lentiviral vector to express MYC Glu39Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120513 | EF1a_MYC Thr58Ala_P2A_Hygro_Barcode | Barcoded lentiviral vector to express MYC Thr58Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 120514 | EF1a_MYC Ser62Ala_P2A_Hygro_Barcode | Barcoded lentiviral vector to express MYC Ser62Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq. |
| 135003 | EF1a_mCherry_P2A_Hygro | Lentiviral vector to express mCherry or to clone and express gene of interest, under control of EF1a promoter. Barcodes compatible with detection in single cell RNA-seq may be inserted at HpaI site. |