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Liquid Nuclear Condensates Mechanically Sense and Restructure the Genome.
Shin Y, Chang YC, Lee DSW, Berry J, Sanders DW, Ronceray P, Wingreen NS, Haataja M, Brangwynne CP
Cell. 2018 Nov 29;175(6):1481-1491.e13. doi: 10.1016/j.cell.2018.10.057.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
121966pHR-scFv-GCN4-sfGFP-GB1-NLS-iLID-dWPREExpresses fusion of single chain variable fragment recognizing SunTag, fluorescent protein sfGFP, and iLID which upon light activation binds to sspB.
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121968pHR-BRD4ΔN-mCherry-sspBExpresses fusion of disordered protein BRD4(462-1362), fluorescent protein mCherry, and sspB which upon light activation binds to iLID.
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122151pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPREExpresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.
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122436pHR-pSFFV-TAF15N-mCherry-sspBExpresses fusion of disordered protein TAF15(1-208), fluorescent protein mCherry, and SspB which upon light activation binds to iLID.
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122439pHR-BRD4ΔN-miRFP670-Cry2WTExpresses disordered protein BRD4(462-1362) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT
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122440pHR-TAF15N-miRFP670-Cry2WTExpresses disordered protein TAF15(1-208) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT
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122441pHR-FUSN-miRFP670-Cry2WTExpresses disordered protein FUS(1-214) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT
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122442pHR-HP1α-miRFP670-Cry2WTExpresses disordered protein HP1α fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT
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122659pLV-sgTelExpresses sgRNA for telomere repeats with fluorescent indicator BFP and puromycin selection marker
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