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A Photoactivatable Botulinum Neurotoxin for Inducible Control of Neurotransmission.
Liu Q, Sinnen BL, Boxer EE, Schneider MW, Grybko MJ, Buchta WC, Gibson ES, Wysoczynski CL, Ford CP, Gottschalk A, Aoto J, Tucker CL, Kennedy MJ
Neuron. 2019 Jan 15. pii: S0896-6273(19)30003-0. doi: 10.1016/j.neuron.2019.01.002.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
122971pQL47 EGFP-VAMP-GSTReporter of botulinum toxin B cleavage
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122981pAAV hSyn syph-GFP-myc-BoNT/B(1-146)-iLIDAAV plasmid with human synapsin promoter driving synaptophysin fused to GFP, iLID(V416I) and BoNT/B amino acids 1-146. Co-express with SSPB-BoNT(147-441, Y365A) for vPA-BoNT
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122982pAAV hSyn mCh-IRES-BoNT/B(1-146)-iLIDAAV plasmid with human synapsin promoter driving mCh and BoNT/B amino acids 1-146 fused to iLID(V416I), separated with an IRES element. Co-express with SSPB-BoNT(147-441, Y365A) for sPA-BoNT
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122985pAAV hSyn mCh-IRES-SspB(milli)-BoNT/B(147-441, Y365A)AAV plasmid with human synapsin promoter driving mCh and SspB(milli)-BoNT/B amino acids 147-441 separated with an IRES element. Co-express with BoNT/B(1-146)-iLID construct for PA-BoNT
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122986pAAV hSyn mCh-IRES-SspB(micro)-BoNT/B(147-441, Y365A)AAV plasmid with human synapsin promoter driving mCh and SspB(micro)-BoNT/B amino acids 147-441 separated with an IRES element. Co-express with BoNT/B(1-146)-iLID construct for PA-BoNT
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