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Lrig1 expression prospectively identifies stem cells in the ventricular-subventricular zone that are neurogenic throughout adult life.
Nam HS, Capecchi MR
Neural Dev. 2020 Mar 17;15(1):3. doi: 10.1186/s13064-020-00139-5.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
125747pCAG-sfGFPsfGFP without iCreERT2 fusion
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125748pCAG-sfGFP-GSAx9-iCre-ERT2sfGFP and iCreERT2 fused by GSAx9 linker
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125749pCAG-sfGFP-iCre-ERT2sfGFP and iCreERT2 fused by unskippable linker
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125797CAG-loxP-3xpA-H2BsfGFP-tdTomato - linker EHistone H2B-superfolder GFP and tdTomato fused by unskippable linker
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126651pB6-Lrig1-T2A-sfGFP-iCre-ERT2-FNF-TKB6J Lrig1-T2A-sfGFP-iCre-ERT2 allele targeting vector, low copy number
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126925CAG-loxP-3xpA-tdTomatotdTomato cre reporter
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128166pCAGGS-mTagBFP2-T2A-sfGFP-iCre-ERT2Monomeric TagBFP2 and sfGFP-iCre-ERT2 recombinase (PAPGSTM N-terminus, unskippable linker)
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128167pCAGGS-mTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2Monomeric TagBFP2 and sfGFP-iCre-ERT2 recombinase (PAPGSTM N-terminus, GSAx9 linker)
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128168pCAGGS-mTagBFP2-T2A-iCre-ERT2Monomeric TagBFP2 and iCre-ERT2 recombinase (PAPGSTMA N-terminus)
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134320pB6-Lrig1-T2A-tdTomato-FNF-TKB6J Lrig1-T2A-tdTomato allele targeting vector, low copy number
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134321pB6-Cdk6-T2A-td-sfGFP-FNF-TKB6J Cdk6-T2A-td-sfGFP allele targeting vector, low copy number
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