A Genetic Tool to Track Protein Aggregates and Control Prion Inheritance.
Newby GA, Kiriakov S, Hallacli E, Kayatekin C, Tsvetkov P, Mancuso CP, Bonner JM, Hesse WR, Chakrabortee S, Manogaran AL, Liebman SW, Lindquist S, Khalil AS
Cell. 2017 Nov 2;171(4):966-979.e18. doi: 10.1016/j.cell.2017.09.041. Epub 2017 Oct 19. PubMed Article
Newby GA, Kiriakov S, Hallacli E, Kayatekin C, Tsvetkov P, Mancuso CP, Bonner JM, Hesse WR, Chakrabortee S, Manogaran AL, Liebman SW, Lindquist S, Khalil AS
Cell. 2017 Nov 2;171(4):966-979.e18. doi: 10.1016/j.cell.2017.09.041. Epub 2017 Oct 19. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 129202 | pGAN147 | yeast transcriptional reporter of aggregating proteins (yTRAP) to detect protein aggregation, Gateway backbone |
| 129203 | pGAN200 | yTRAP sensor of [PSI+], detects aggregation of Sup35 |
| 129204 | pGAN201 | yTRAP sensor of [RNQ+], detects aggregation of Rnq1 |
| 129249 | pSK221 | reverse yTRAP, tetR-responsive Neon reporter |
| 129252 | pSK260 | dual yTRAP, NM fusion to aTF1 reporting on [PSI+] state with mKate2 reporter |
| 129268 | pSK226 | dual yTRAP, RNQ1 fusion to aTF2 reporting on [RNQ+] state with mNeonGreen reporter |
| 129270 | pGAN213 | Control for measuring background levels of yTRAP reporter protein |
| 129272 | pSK267 | "ZF only" yTRAP control to control for yTRAP reporter fluorescence changes not dependent on sensed protein |
| 131122 | pGAN230 | reverse yTRAP, RNQ1-aTF reporting on tetR-mKate2 |
| 131123 | pGAN101 | Plasmid for overexpression of prion domains to induce prions |
| 131148 | pSK247 | dual yTRAP, Gateway destination vector of aTF2 fusion reporting on mNeonGreen |
| 131149 | pSK234 | dual yTRAP, Gateway destination vector of aTF2 fusion reporting on mKate2 |