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Generation of synthetic nanobodies against delicate proteins.
Zimmermann I, Egloff P, Hutter CAJ, Kuhn BT, Brauer P, Newstead S, Dawson RJP, Geertsma ER, Seeger MA
Nat Protoc. 2020 Apr 8. pii: 10.1038/s41596-020-0304-x. doi: 10.1038/s41596-020-0304-x.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
132695pRDV5 containing Sb-looppRDV5 plasmid containing a loop Sybody for the generation of a qPCR standard curve
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132696pRDV5 containing Sb-convexpRDV5 plasmid containing a convex Sybody for the generation of a qPCR standard curve
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132697pDX_init containing Sb-looppDX_init plasmid containing a loop Sybody for the generation of a qPCR standard curve
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132698pSb_init containing the TM287/288 nanobody Nb_TM#2expresses the TM287/288 nanobody Nb_TM#2 in E.coli, to use as positive control in ELISA
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132699pSb_init containing the MBP sybody Sb_MBP#1expresses the MBP sybody Sb_MBP#1 in E.coli, to use as positive control in ELISA
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132700pBXNH3CA_MBPexpresses Avi-tagged MBP in E.coli, to use as positive control in ELISA
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132701pBXNH3LCA_TM287/288expresses TM287/288 in E.coli, to use as positive control in ELISA
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