De novo-designed translation-repressing riboregulators for multi-input cellular logic.
Kim J, Zhou Y, Carlson PD, Teichmann M, Chaudhary S, Simmel FC, Silver PA, Collins JJ, Lucks JB, Yin P, Green AA
Nat Chem Biol. 2019 Dec;15(12):1173-1182. doi: 10.1038/s41589-019-0388-1. Epub 2019 Nov 4. PubMed Article
Kim J, Zhou Y, Carlson PD, Teichmann M, Chaudhary S, Simmel FC, Silver PA, Collins JJ, Lucks JB, Yin P, Green AA
Nat Chem Biol. 2019 Dec;15(12):1173-1182. doi: 10.1038/s41589-019-0388-1. Epub 2019 Nov 4. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 132722 | pYZ_3WJrep_N20_switch | T7 RNAP-driven expression of 3WJ repressor index 20 switch RNA with a GFP-ASV reporter |
| 132723 | pYZ_3WJrep_N19_switch | T7 RNAP-driven expression of 3WJ repressor index 19 switch RNA with a GFP-ASV reporter |
| 132724 | pYZ_3WJrep_N10_switch | T7 RNAP-driven expression of 3WJ repressor index 10 switch RNA with a GFP-ASV reporter |
| 132725 | pYZ_3WJrep_N24_switch | T7 RNAP-driven expression of 3WJ repressor index 24 switch RNA with a GFP-ASV reporter |
| 132726 | pYZ_3WJrep_N20_trigger | T7 RNAP-driven expression of 3WJ repressor index 20 trigger RNA |
| 132727 | pYZ_3WJrep_N19_trigger | T7 RNAP-driven expression of 3WJ repressor index 19 trigger RNA |
| 132728 | pYZ_3WJrep_N10_trigger | T7 RNAP-driven expression of 3WJ repressor index 10 trigger RNA |
| 132729 | pYZ_3WJrep_N24_trigger | T7 RNAP-driven expression of 3WJ repressor index 24 trigger RNA |
| 132730 | pYZ_NAND2_L17_S19_S11 | T7 RNAP-driven expression of a 2-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 19 and 11 |
| 132731 | pYZ_NAND3_L11_S11_S13_S19 | T7 RNAP-driven expression of a 3-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 11, 13, and 19 |
| 132732 | pYZ_NAND4_L17_S24_S11_S19_S13 | T7 RNAP-driven expression of a 4-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 24, 11, 19, and 13 |
| 132733 | pYZ_3WJrep_N11_trigger | T7 RNAP-driven expression of 3WJ repressor index 11 trigger RNA |
| 132734 | pYZ_3WJrep_N12_trigger | T7 RNAP-driven expression of 3WJ repressor index 12 trigger RNA |
| 132735 | pYZ_3WJrep_N13_trigger | T7 RNAP-driven expression of 3WJ repressor index 13 trigger RNA |
| 132736 | pAG_PluxB_ToeRep_N01_trigger | Inducible expression of first-generation toehold repressor index 1 trigger RNA via the PluxB promoter |
| 132737 | pAG_Ptet*_ToeRep_N01_switch | Inducible expression of first-generation toehold repressor index 1 switch RNA with a GFP-ASV reporter via the Ptet* promoter |
| 132738 | pYZ_PluxB_3WJrep_N19_trigger | Inducible expression of 3WJ repressor index 19 trigger RNA via the PluxB promoter |
| 132739 | pYZ_Ptet*_3WJrep_N19_switch | Inducible expression of 3WJ repressor index 19 switch RNA with a GFP-ASV reporter via the Ptet* promoter |
| 132740 | pAG_ToeRep_N09_trigger | T7 RNAP-driven expression of first-generation toehold repressor index 9 trigger RNA |
| 132741 | pAG_ToeRep_N09_switch | T7 RNAP-driven expression of first-generation toehold repressor index 9 switch RNA with a GFP-ASV reporter |
| 132742 | pJK_ToeRepG2_N02_switch | T7 RNAP-driven expression of second-generation toehold repressor index 2 switch RNA with a GFP-ASV reporter |
| 132743 | pJK_ToeRepG2_N64_switch | T7 RNAP-driven expression of second-generation toehold repressor index 64 switch RNA with a GFP-ASV reporter |
| 132744 | pJK_ToeRepG2_N19_switch | T7 RNAP-driven expression of second-generation toehold repressor index 19 switch RNA with a GFP-ASV reporter |
| 132745 | pJK_ToeRepG2_N02_trigger | T7 RNAP-driven expression of second-generation toehold repressor index 2 trigger RNA |
| 132746 | pJK_ToeRepG2_N64_trigger | T7 RNAP-driven expression of second-generation toehold repressor index 64 trigger RNA |
| 132747 | pJK_ToeRepG2_N19_trigger | T7 RNAP-driven expression of second-generation toehold repressor index 19 trigger RNA |