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Developmental analyses of mouse embryos and adults using a non-overlapping tracing system for all three germ layers.
Serizawa T, Isotani A, Matsumura T, Nakanishi K, Nonaka S, Shibata S, Ikawa M, Okano H
Development. 2019 Nov 4;146(21). pii: dev.174938. doi: 10.1242/dev.174938.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
140759ROSA26(MultiFPsĪ”Puro)Targeting vector to Gt(ROSA)26 locus for conditional expression of distinct FPs (Venus, mCherry, and mCerulean) responding to each site-specific-recombinase activity (Cre, Dre, and phiC31o).
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140760Sox1(2A-Cre)Targeting vector to Sox1 gene locus for induction of P2A-fused Cre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(WT) sequence.
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140761T/brachyury(2A-Dre)Targeting vector to T/brachyury gene locus for induction of P2A-fused Dre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F3) sequence.
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140762Sox17(2A-phiC31o)Targeting vector to Sox17 gene locus for induction of P2A-fused phiC31o, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F5) sequence.
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