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ROSA26(MultiFPsΔPuro)
(Plasmid #140759)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 140759 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCAGGS
  • Backbone size w/o insert (bp) 2396
  • Total vector size (bp) 18841
  • Vector type
    Mouse Targeting
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    puromycin-N-acetyltransferase
  • Alt name
    Pac
  • Species
    Synthetic
  • Insert Size (bp)
    600
  • Promoter CAGGS

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Venus
  • Alt name
    YFP
  • Alt name
    GFP variant
  • Species
    Synthetic
  • Insert Size (bp)
    720
  • Promoter CAGGS

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    mCherry
  • Alt name
    RFP variant
  • Species
    Synthetic
  • Insert Size (bp)
    708
  • Promoter CAGGS

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    mCerulean
  • Alt name
    CFP
  • Alt name
    GFP variant
  • Species
    Synthetic
  • Insert Size (bp)
    720
  • Promoter CAGGS

Cloning Information for Gene/Insert 4

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Venus was cloned from pTP1-Venus (Kohyama et al., Dev. Biol. 286, 311-325, 2005); mCherry was cloned from pG-PB-Zscan4c-mCherry-polyAfloxPGKneo, a gift from Minoru Ko, Keio University School of Medicine, Japan; mCerulean was cloned from Cerulean (Addgene plasmid #15214, Rizzo et al., Nat. Biotechnol. 22, 445-449, 2004), a gift from Dave Piston, Washington University School of Medicine, USA.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ROSA26(MultiFPsΔPuro) was a gift from Hideyuki Okano (Addgene plasmid # 140759 ; http://n2t.net/addgene:140759 ; RRID:Addgene_140759)
  • For your References section:

    Developmental analyses of mouse embryos and adults using a non-overlapping tracing system for all three germ layers. Serizawa T, Isotani A, Matsumura T, Nakanishi K, Nonaka S, Shibata S, Ikawa M, Okano H. Development. 2019 Nov 4;146(21). pii: dev.174938. doi: 10.1242/dev.174938. 10.1242/dev.174938 PubMed 31597657