Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

NL4-3 mCherry Luciferase
(Plasmid #44965)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 44965 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pNLENG1
  • Backbone manufacturer
    AIDS Research and Reference Reagent Program
  • Total vector size (bp) 12424
  • Vector type
    Lentiviral
  • Selectable markers
    mCherry, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mCherry, Luciferase, IRES
  • Alt name
    HIV 1 reporter virus
  • Species
    Lentivirus, Human specific
  • Mutation
    S34C mutation in Nef
  • Promoter EF-1 alpha– driven mCherry:Puromycin
  • Tags / Fusion Proteins
    • mCherry with D212G (C terminal on insert)
    • Luciferase, IRES (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (destroyed during cloning)
  • 5′ sequencing primer from pNLENG1 5'
  • 3′ sequencing primer from pNLENG1 3'
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please note that the D212G mutation in mCherry has no effect on the function of this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NL4-3 mCherry Luciferase was a gift from Warner Greene (Addgene plasmid # 44965 ; http://n2t.net/addgene:44965 ; RRID:Addgene_44965)
  • For your References section:

    A flexible model of HIV-1 latency permitting evaluation of many primary CD4 T-cell reservoirs. Lassen KG, Hebbeler AM, Bhattacharyya D, Lobritz MA, Greene WC. PLoS One. 2012;7(1):e30176. doi: 10.1371/journal.pone.0030176. Epub 2012 Jan 24. 10.1371/journal.pone.0030176 PubMed 22291913