Systematic tools for reprogramming plant gene expression in a simple model, Marchantia polymorpha.
Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, Markel K, Delmans M, West A, Patron NJ, Haseloff J
ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511. PubMed Article
Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, Markel K, Delmans M, West A, Patron NJ, Haseloff J
ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 136067 | pCsA | Acceptor plasmid for even level position 1, lacZ blue-white screening |
| 136068 | pCsB | Acceptor plasmid for even level position 2, lacZ blue-white screening |
| 136069 | pCsC | Acceptor plasmid for even level position 3, lacZ blue-white screening |
| 136070 | pCsE | Acceptor plasmid for even level position 4, lacZ blue-white screening |
| 136071 | pCk1_spacer | Use when making even level constructs (L2,...) if pCk1 position (position 1) has no construct assigned |
| 136072 | pCk2_spacer | Use when making even level constructs (L2,...) if pCk2 position (position 2) has no construct assigned |
| 136073 | pCk3_spacer | Use when making even level constructs (L2,...) if pCk3 position (position 3) has no construct assigned |
| 136074 | pCk4_spacer | Use when making even level constructs (L2,...) if pCk4 position (position 4) has no construct assigned |
| 136075 | pCsA_spacer | Use when making odd level constructs (L3,...) if pCsA position (position 1) has no construct assigned |
| 136076 | pCsB_spacer | Use when making odd level constructs (L3,...) if pCsB position (position 2) has no construct assigned |
| 136077 | pCsC_spacer | Use when making odd level constructs (L3,...) if pCsC position (position 3) has no construct assigned |
| 136078 | pCsE_spacer | Use when making odd level constructs (L3,...) if pCsE position (position 4) has no construct assigned |
| 136079 | pUAP4 | Acceptor plasmid for L0 parts. Clone L0 parts into pUAP4 with SapI, lacZ blue-white screening. Release parts to assemble transcription units with BsaI. |
| 136080 | CDS_nptII | Confers plant resistance to kanamycin |
| 136081 | CDS_mALS | Confers plant resistance to chlorsulfuron |
| 136082 | CDS_hptII | Confers plant resistance to hygromycin |
| 136083 | CDS_LUC | Firefly luciferase |
| 136084 | CDS_GUS | For histochemical _-glucuronidase activity assays. This GUS CDS does not have an intron. |
| 136085 | CDS12_eGFP | FP for N-term fusion with a CTAG |
| 136086 | CDS12_eGFP-linker | FP for N-term fusion with a CTAG without linker |
| 136087 | CDS12_mScarlet-I | FP for N-term fusion with a CTAG |
| 136088 | CDS12_mScarletI-linker | FP for N-term fusion with a CTAG without linker |
| 136089 | CDS12_mTurquoise2 | FP for N-term fusion with a CTAG |
| 136090 | CDS12_mTurquoise2-linker | FP for N-term fusion with a CTAG without linker |
| 136091 | CDS12_mVenus | FP for N-term fusion with a CTAG |
| 136092 | CDS12_mVenus-linker | FP for N-term fusion with a CTAG without linker |
| 136093 | CDS12_TP-MpSIG2 | Chloroplast transit peptide from MpSIG2. For N-term fusion with a CTAG |
| 136094 | CDS12-MpER-Targ | CDS12 with ER targeting peptide to fuse with CTAG containing HDEL ER retention signal at the 3'end |
| 136095 | CTAG_eGFP | FP for C-term fusion with CDS12 |
| 136096 | CTAG_eGFP-GHtag-HDEL | FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide. Contains Gly-His tag |
| 136097 | CTAG_eGFP-HDEL | FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide |
| 136098 | CTAG_linker-eGFP | FP for C-term fusion with CDS12 without linker |
| 136099 | CTAG_linker-Lti6b | Plasma Membrane targeting protein for C-term fusion with CDS12 without linker. |
| 136100 | CTAG_linker-mTurquoise2 | FP for C-term fusion with CDS12 without linker |
| 136101 | CTAG_linker-mVenus | FP for C-term fusion with CDS12 without linker |
| 136102 | CTAG_linker-N7 | Nuclear targeting peptide for C-term fusion with CDS12 without linker. |
| 136103 | 5UTR_MpNOP1 | 5UTR for cloning with PROM_MpNOP1 |
| 136104 | CTAG_mTurquoise2 | FP for C-term fusion with CDS12 |
| 136105 | CTAG_mTurquoise2-HDEL | FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide |
| 136106 | CTAG_mVenus | FP for C-term fusion with CDS12 |
| 136107 | CTAG_mVenus-HDEL | FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide |
| 136108 | CTAG_NLS-SV40 | CTAG Nuclear localiation signal SV40 for fusion with CDS12 |
| 136109 | PROM_MpUBE2 | PROM for cloning with 5UTR_MpUBE2. PROM+5UTR_MpUBE2 has ubiquitous expression in early satges of gemmae development. |
| 136110 | 5UTR_MpUBE2 | 5UTR for cloning with PROM_MpUEB2. PROM+5UTR_MpUBE2 has ubiquitous expression in early stages of gemmae development. |
| 136111 | PROM5_35S | Strong promoter that drives expression everywhere in Mp gemmae but much weaker in the notches |
| 136112 | PROM5_35Sx2 | Strong promoter that drives expression everywhere in Mp gemmae but much weaker in the notches |
| 136113 | PROM5_MpEF1a | Strong promoter from MpEF1a, that drives expression everywhere in Mp gemmae but stronger in the notches |
| 136114 | 3TERM_35S | 3TERM after CDS/ CDS12-CTAG |
| 136115 | 3TERM_Nos | 3TERM after CDS/ CDS12-CTAG |
| 136116 | 3TERM_Nos-35S | 3TERM after CDS/ CDS12-CTAG |
| 136117 | PROM_MpNOP1 | PROM for cloning with 5UTR_MpNOP1 |
| 136118 | CTAG_mScarlet-I | FP for C-term fusion with CDS12 |
| 136119 | 3TERM_MpNOP1 | 3TERM after CDS/ CDS12-CTAG |
| 136120 | PROM5_MpU6 | Mp U6 promoter (type III RNA polymerase promoter) to drive expression of gRNA for CRISPR/Cas9 |
| 136121 | CDS_Cas9-NLS | Puchta Arabidopsis codon-optimised Cas9 for CRISPR |
| 136122 | CDS12_Cas9-NLS | Puchta Arabidopsis codon-optimised Cas9 for CRISPR. CDS12 for N-term fusion with a CTAG |
| 136123 | L1_KanR-Ck1 | L1 in position 1 (next to LB in L2 construct), for KanR |
| 136124 | L1_CsR-Ck1 | L1 in position 1 (next to LB in L2 construct), for CsR |
| 136125 | L1_HyR-Ck1 | L1 in position 1 (next to LB in L2 construct), for HygR |
| 136126 | L1_NOP1:mS-N7-Ck2 | L1 in position 2, example of weak promoter |
| 136127 | L1_UBE2:mT-N7-Ck2 | L1 in position 2, control of expression |
| 136128 | L1_UBE2:mT-N7-Ck3 | L1 in position 3, control of expression |
| 136129 | L1_UBE2:eG-Lt-Ck3 | L1 in position 3, mark cell outline with eGFP |
| 136130 | L1_UBE2:eG-Lt-Ck4 | L1 in position 4, mark cell outline with eGFP |
| 136131 | L1_UBE2:mS-Lt-Ck3 | L1 in position 3, mark cell outline with mScarletI |
| 136132 | L1_UBE2:mS-Lt-Ck4 | L1 in position 4, mark cell outline with mScarletI |
| 136133 | L2_UBE2:mT-N7-CsA | Plasmid to transform Mp with constitutive promoter driving mTurq to the nuclei. HygR |
| 136134 | L2_UBE2:eG-Lt-CsA | Plasmid to transform Mp with constitutive promoter marking cell outline with eGFP. HygR |
| 136135 | L1_Cas9-Ck4 | L1 in position 4, for AtcoCas9 (Puchta) expression in L2 CRISPR vectors |
| 136136 | L1_lacZgRNA-Ck3 | L1 in position 3, to clone gRNA target using BbsI, lacZ blue-white screening |
| 136137 | L1_lacZgRNA-Ck2 | L1 in position 2, to clone gRNA target using BbsI, lacZ blue-white screening |
| 136138 | L2_lacZgRNA-Cas9-CsA | Plasmid to accept gRNA target with SapI, lacZ blue-white screening. Contains Cas9 and HygR. |
| 136139 | L2_NOP1gRNA-Cas9-CsA | Plasmid with NOP1-gRNA to tranform Mp as CRISPR control. Contains Cas9 and HygR |
| 136140 | L2_2xNOP1gRNA-Cas9-CsA | Plasmid with 2 different NOP1-gRNA. Targets 500 bp apart in the genome. To tranform Mp as CRISPR control. Contains Cas9 and HygR |
| 136695 | pCk1 | Acceptor plasmid for odd level position 1, lacZ blue-white screening. Part of the OpenPlant toolkit. |
| 136696 | pCk2 | Acceptor plasmid for odd level position 2, lacZ blue-white screening. Part of the OpenPlant toolkit. |
| 136697 | pCk3 | Acceptor plasmid for odd level position 3, lacZ blue-white screening. Part of the OpenPlant toolkit. |
| 136698 | pCk4 | Acceptor plasmid for odd level position 4, lacZ blue-white screening. Part of the OpenPlant toolkit. |
| 163931 | 3TERM_bba_b0015_rev_chlo | L0 part - terminator |
| 163932 | 3TERM_Ntpsba_rev_chlo | L0 part - terminator |
| 163935 | CDS_aadA_chlo | L0 part - CDS |
| 163936 | CDS_mTurqcp_chlo | L0 part - CDS |
| 163937 | L1-Ck1-hs1-l-chlo | For integration in the chloroplast trnG_trnfM region: HS1-L, genome position: 40216_ 41843, Genbank accession no. MH635409 |
| 163938 | L1-Ck1-hs2-l-chlo | For integration in the chloroplast rbcL_trnR region: HS2-L, genome position: 56139_57662, Genbank accession no. MH635409 |
| 163939 | L1-Ck2-smR-chlo | L1 in position 2, for spectinomycin in chloroplast transformation |
| 163940 | L1-Ck4-hs1-r-chlo | For integration in the chloroplast trnG_trnfM region: HS1-R, genome position: 41847_ 43622, Genbank accession no. MH635409 |
| 163941 | L1-Ck4-hs2-r-chlo | For integration in the chloroplast rbcL_trnR region: HS2-R, genome position: 57681- 59304, Genbank accession no. MH635409 |
| 163942 | pCk1chlo | Acceptor plasmid for odd level position 1 |
| 163943 | pCk2chlo | Acceptor plasmid for odd level position 2 |
| 163944 | pCk3chlo | Acceptor plasmid for odd level position 3 |
| 163945 | pCk4chlo | Acceptor plasmid for odd level position 4 |
| 163946 | pCsAchlo | Acceptor plasmid for even level position 1 |
| 163947 | pCsBchlo | Acceptor plasmid for even level position 2 |
| 163948 | pCsCchlo | Acceptor plasmid for even level position 3 |
| 163949 | pCsEchlo | Acceptor plasmid for even level position 4 |
| 163951 | PROM5_Mprnpo_chlo | L0 part - promoter |
| 163952 | PROM5_Ntprrn_rev_chlo | L0 part - promoter |
| 163953 | PROM5_Ntpsba_chlo | L0 part - promoter |