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Spatiotemporal control of phosphatidic acid signaling with optogenetic, engineered phospholipase Ds.
Tei R, Baskin JM
J Cell Biol. 2020 Mar 2;219(3). pii: 133675. doi: 10.1083/jcb.201907013. PubMed Article

Plasmids from Article

ID Plasmid Purpose
140055Endosome-targeted optodPLDEncodes CRY2-mCherry-dPLD-P2A-CIBN-2xFYVE for mammalian expression
140056Endosome-targeted optoPLDEncodes CRY2-mCherry-PLD-P2A-CIBN-2xFYVE for mammalian expression
140057Golgi-targeted optodPLDEncodes CRY2-mCherry-dPLD-P2A-CIBN-Sialyltransferase(TM) for mammalian expression
140058Golgi-targeted optoPLDEncodes CRY2-mCherry-PLD-P2A-CIBN-Sialyltransferase(TM) for mammalian expression
140059Endoplasmic reticulum-targeted optodPLDEncodes CRY2-mCherry-dPLD-P2A-CIBN-Sac1(TM) for mammalian expression
140060Endoplasmic reticulum-targeted optoPLDEncodes CRY2-mCherry-PLD-P2A-CIBN-Sac1(TM) for mammalian expression
140061Plasma membrane-targeted optodPLDEncodes CRY2-mCherry-dPLD-P2A-CIBN-CAAX for mammalian expression
140114Plasma membrane-targeted optoPLDEncodes CRY2-mCherry-PLD-P2A-CIBN-CAAX for mammalian expression

Antibodies from Article