Congenital disorder of glycosylation caused by starting site-specific variant in syntaxin-5.
Linders PTA, Gerretsen ECF, Ashikov A, Vals MA, de Boer R, Revelo NH, Arts R, Baerenfaenger M, Zijlstra F, Huijben K, Raymond K, Muru K, Fjodorova O, Pajusalu S, Ounap K, Ter Beest M, Lefeber D, van den Bogaart G
Nat Commun. 2021 Oct 28;12(1):6227. doi: 10.1038/s41467-021-26534-y. PubMed Article
Linders PTA, Gerretsen ECF, Ashikov A, Vals MA, de Boer R, Revelo NH, Arts R, Baerenfaenger M, Zijlstra F, Huijben K, Raymond K, Muru K, Fjodorova O, Pajusalu S, Ounap K, Ter Beest M, Lefeber D, van den Bogaart G
Nat Commun. 2021 Oct 28;12(1):6227. doi: 10.1038/s41467-021-26534-y. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 154845 | str-KDEL_Stx5S-SBP-mCitrine | Synchronize trafficking of Stx5S from the ER, for use with FLIM (RUSH system) |
| 154846 | str-KDEL_Stx5LdeltaER-SBP-mCitrine | Synchronize trafficking of Stx5LdeltaER (M55V) from the ER, for use with FLIM (RUSH system) |
| 165082 | pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B1 | gRNA 1 of pair B for Stx5L-specific knockout |
| 165083 | pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B2 | gRNA 2 of pair B for Stx5L-specific knockout |
| 165085 | pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C1 | gRNA 1 of pair C for Stx5L-specific knockout |
| 165086 | pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C2 | gRNA 2 of pair C for Stx5L-specific knockout |
| 165087 | Stx5S-mCherry | Expresses Stx5S-mCherry in mammalian cells |
| 209844 | Stx5L-mCherry | Expresses Stx5L-mCherry in mammalian cells |
| 209845 | Stx5L(m55V)-GFP | Expresses Stx5 M55V mutant fused to GFP in mammalian cells |
| 209846 | str-KDEL_Stx5L(M55V)-SBP-mCitrine | Synchronize trafficking of Stx5L (M55V) from the ER, for use with FLIM (RUSH system) |
| 209847 | Stx5S-GFP | Expresses Stx5S-GFP in mammalian cells |
| 209848 | Bet1-mCherry | Expresses Bet1-mCherry in mammalian cells |
| 209849 | Bet1L-mCherry | Expresses Bet1L-mCherry in mammalian cells |
| 209850 | Bet1-GFP | Expresses Bet1-GFP in mammalian cells |