Revealing the Activity of Trimeric G-proteins in Live Cells with a Versatile Biosensor Design.
Maziarz M, Park JC, Leyme A, Marivin A, Garcia-Lopez A, Patel PP, Garcia-Marcos M
Cell. 2020 Jun 27. pii: S0092-8674(20)30752-2. doi: 10.1016/j.cell.2020.06.020. PubMed Article
Maziarz M, Park JC, Leyme A, Marivin A, Garcia-Lopez A, Patel PP, Garcia-Marcos M
Cell. 2020 Jun 27. pii: S0092-8674(20)30752-2. doi: 10.1016/j.cell.2020.06.020. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 158090 | pcDNA3.1-Gαi*-BERKY1 | BRET biosensor for detection of Gαi-GTP in mammalian expression vector pcDNA3.1(+) |
| 158197 | pcDNA3.1-Gαi*-BERKY2 | BRET biosensor for detection of Gαi-GTP in mammalian expression vector pcDNA3.1(+) |
| 158198 | pcDNA3.1-Gαi*-BERKY3 | BRET biosensor for detection of Gαi-GTP in mammalian expression vector pcDNA3.1(+) |
| 158217 | pcDNA3.1-Gαq*-BERKY3 | BRET biosensor for detection of Gαq-GTP in mammalian expression vector pcDNA3.1(+) |
| 158218 | pcDNA3.1-Gα13*-BERKY3 | BRET biosensor for detection of Gα13-GTP in mammalian expression vector pcDNA3.1(+) |
| 158219 | pcDNA3.1-Gβγ-BERKY3 | BRET biosensor for detection of free Gβγ in mammalian expression vector pcDNA3.1(+) |
| 158220 | pcDNA3.1-Rho*-BERKY3 | BRET biosensor for detection of Rho-GTP in mammalian expression vector pcDNA3.1(+) |
| 158424 | pLVX-IRES-Gαi*-BERKY3 | BRET biosensor for detection of Gαi-GTP in lentiviral vector pLVX-IRES-Hyg |
| 158425 | pLVX-IRES-Gαq*-BERKY3 | BRET biosensor for detection of Gαq-GTP in lentiviral vector pLVX-IRES-Hyg |
| 158426 | pLVX-IRES-Gβγ-BERKY3 | BRET biosensor for detection of free Gβγ in lentiviral vector pLVX-IRES-Hyg |
| 158427 | pLenti-hSyn-Gαi*-BERKY1 | BRET biosensor for detection of Gαi-GTP in lentiviral vector pLenti-hSyn |
| 158428 | pLenti-hSyn-Gαi*-BERKY3 | BRET biosensor for detection of Gαi-GTP in lentiviral vector pLenti-hSyn |
| 158429 | pLenti-hSyn-Gβγ-BERKY1 | BRET biosensor for detection of free Gβγ in lentiviral vector pLenti-hSyn |
| 158430 | pLenti-hSyn-Gαq*-BERKY1 | BRET biosensor for detection of Gαq-GTP in lentiviral vector pLenti-hSyn |
| 158603 | pcDNA3.1-mas-KB-1753-Nluc | Plasmid expressing KB-1753-Nluc in mammalian cells |
| 158604 | pcDNA3.1-mas-KB-1753-Nluc I9A/W10A | Plasmid expressing KB-1753-Nluc I9A/W10A in mammalian cells |
| 158605 | pcDNA3.1-mas-GRK2RH-Nluc | Plasmid expressing GRK2(RH)-Nluc in mammalian cells |
| 158606 | pcDNA3.1-mas-GRK2RH-Nluc L118A | Plasmid expressing GRK2(RH)-Nluc L118A in mammalian cells |
| 158607 | pcDNA3.1-mas-p115RH-Nluc | Plasmid expressing p115(RH)-Nluc in mammalian cells |
| 158608 | pcDNA3.1-mas-PRGRH-Nluc | Plasmid expressing PRG(RH)-Nluc in mammalian cells |
| 171374 | pcDNA3.1-Gα13(EE)-YFP | Plasmid encoding Gα13 internally-tagged with the Citrine variant of YFP in the b/c loop |