Overcoming the design, build, test bottleneck for synthesis of nonrepetitive protein-RNA cassettes.
Katz N, Tripto E, Granik N, Goldberg S, Atar O, Yakhini Z, Orenstein Y, Amit R
Nat Commun. 2021 Mar 11;12(1):1576. doi: 10.1038/s41467-021-21578-6. PubMed Article
Katz N, Tripto E, Granik N, Goldberg S, Atar O, Yakhini Z, Orenstein Y, Amit R
Nat Commun. 2021 Mar 11;12(1):1576. doi: 10.1038/s41467-021-21578-6. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 158199 | CMV-10xPP7 oligo-library based | Cassette of 10 different binding sites with high affinity to PP7 phage coat protein, as extracted from the oligo pool experiment. |
| 158200 | CMV-10xMS2 oligo-library based | Cassette of 10 different binding sites with high affinity to MS2 phage coat protein, as extracted from the oligo pool experiment. |
| 158201 | CMV-10xQb oligo-library based | Cassette of 10 different binding sites with high affinity to QB phage coat protein, as extracted from the oligo pool experiment. |
| 158203 | CMV-10xPP7-Qb model based | Cassette of 10 different binding sites with high affinity to both PP7 and Qb phage coats protein, as computed by the ML model. |
| 158204 | CMV-10xMS2 no wild type | Cassette of 10 different binding sites with high affinity to MS2 phage coat protein, as extracted form the oligo pool experiment. It does not contain the MS2-wt sequence, therefore QCP cannot bind it. |
| 158205 | CMV-10xQb no wild type | Cassette of 10 different binding sites with high affinity to Qb phage coat protein, as extracted form the oligo pool experiment. It does not contain the Qb-wt sequence, therefore MCP cannot bind it. |
| 158206 | Ef1a-QCP-3xBFP | Qb coat protein fused to three repeats of the blue fluorescent protein under a Efla promoter. |