Kathleen Postle Lab:
Postle Lab Plasmids
Unpublished
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 160815 | pKP1469 | Expression of ExbB protein; the exbB gene replaces tonB gene in BamHI site of pKP325; includes pBAD promoter and optimized RBS from pBAD24-derived pKP660 (ExbB, ExbD) |
| 167593 | pKP372 | Express TonB frameshift of amphipathic helix |
| 167594 | pKP476 | Express TonB ∆(199-216) |
| 167596 | pKP1963 | Express ExbB delta(195-204),ExbD |
| 167597 | pKP1964 | Express ExbB delta(205-214),ExbD |
| 167598 | pKP1965 | Express ExbB delta(215-224),ExbD |
| 167599 | pKP1940 | Express ExbB delta(225-234),ExbD |
| 167600 | pKP1912 | Express ExbB delta(235-244),ExbD |
| 167601 | pKP2008 | Express ExbB (205-214 Ala),ExbD |
| 167602 | pKP2009 | Express ExbB (215-224 Ala),ExbD |
| 167603 | pKP2010 | Express ExbB (235-244 Ala),ExbD |
| 167604 | pKP2019 | Express ExbB (195-204 Ala),ExbD |
| 167605 | pKP2025 | Express ExbB (225-234 Ala),ExbD |
| 167606 | pKP2085 | Express ColicinB-V5-TEV-T7-mature MBP |
| 167615 | pKP1362 | Express TonB C18G. |
| 167616 | pKP1427 | Express TonB C18G, Q160C. |
| 167617 | pKP1708 | Express TonB C18G, H20A, Q160C. |
| 167618 | pKP1676 | Express TonB C18G, M201C. |
| 167619 | pKP1720 | Express TonB C18G, H20A, M201C. |
| 167620 | pKP1867 | Express TonB C18G, R204C. |
| 167621 | pKP1868 | Express TonB C18G, H20A, R204C. |
| 167622 | pKP1683 | Express TonB C18G, V206C. |
| 167623 | pKP1722 | Express TonB C18G, H20A, V206C. |
| 167624 | pKP1646 | Express TonB C18G, N208C. |
| 167625 | pKP1692 | Express TonB C18G, H20A, N208C. |
| 167626 | pKP1647 | Express TonB C18G, A209C. |
| 167627 | pKP2299 | Express TonB C18G, H20A, A209C. |
| 167628 | pKP1624 | Express TonB C18G, R212C. |
| 167629 | pKP1861 | Express TonB C18G, H20A, R212C. |
| 167630 | pKP1684 | Express TonB C18G, R214C. |
| 167631 | pKP1723 | Express TonB C18G, H20A, R214C. |
| 167632 | pKP2303 | Express TonB C18G, Q160C, F202A. |
| 167633 | pKP2304 | Express TonB C18G, Q160C, F202A, W213A. |
| 167634 | pKP1410 | Express FepA D12C . Please note that mutation numbers are not based on start codon of insert. |
| 167635 | pKP2185 | Express FepA D12C, I14P . Please note that mutation numbers are not based on start codon of insert. |
| 167636 | pKP718 | Express FepA T13C . Please note that mutation numbers are not based on start codon of insert. |
| 167637 | pKP2186 | Express FepA T13C, I14P . Please note that mutation numbers are not based on start codon of insert. |
| 167638 | pKP1411 | Express FepA I14C . Please note that mutation numbers are not based on start codon of insert. |
| 167639 | pKP1383 | Express FepA V15C . Please note that mutation numbers are not based on start codon of insert. |
| 167640 | pKP2187 | Express FepA V15C, I14P . Please note that mutation numbers are not based on start codon of insert. |
| 167641 | pKP1384 | Express FepA V16C . Please note that mutation numbers are not based on start codon of insert. |
| 167642 | pKP2188 | Express FepA V16C, I14P . Please note that mutation numbers are not based on start codon of insert. |
| 167643 | pKP1416 | Express FepA T17C . Please note that mutation numbers are not based on start codon of insert. |
| 167644 | pKP2189 | Express FepA T17C, I14P . Please note that mutation numbers are not based on start codon of insert. |
| 167645 | pKP1583 | Express FepA D34C . Please note that mutation numbers are not based on start codon of insert. |
| 167646 | pKP1626 | Express FepA D34C, delta12-16 (TonB box) . Please note that mutation numbers are not based on start codon of insert. |
| 167647 | pKP1382 | Express FepA G54C . Please note that mutation numbers are not based on start codon of insert. |
| 167648 | pKP1627 | Express FepA G54C, delta12-16 (TonB box) . Please note that mutation numbers are not based on start codon of insert. |
| 167649 | pKP1392 | Express ExbD T42C protein |
| 167650 | pKP2040 | Express His6-ExbB S80A, S82A, S84A |
| 167651 | pKP2032 | Express His6-ExbB S43A, S45A, S67A, S80A, S82A, S84A, S98A, S101A, T111A, S112A, S221A, S229A |
| 167652 | pKP932 | Express colicinB-His6, M261C |
| 167653 | pKP968 | Express colicinB-His6, D17C, M261C |
| 167654 | pKP996 | Express colicinB-His6, T18C, M261C |
| 167655 | pKP995 | Express colicinB-His6, M19C, M261C |
| 167656 | pKP997 | Express colicinB-His6, V20C, M261C |
| 167657 | pKP933 | Express colicinB-His6, V21C, M261C |
| 167658 | pKP864 | Express colicinB-His6 |
| 167660 | pKP2264 | Express colicinB-His6, A121C, Y254C |