In vivo screens using a selective CRISPR antigen removal lentiviral vector system reveal immune dependencies in renal cell carcinoma.
Dubrot J, Lane-Reticker SK, Kessler EA, Ayer A, Mishra G, Wolfe CH, Zimmer MD, Du PP, Mahapatra A, Ockerman KM, Davis TGR, Kohnle IC, Pope HW, Allen PM, Olander KE, Iracheta-Vellve A, Doench JG, Haining WN, Yates KB, Manguso RT
Immunity. 2021 Jan 20. pii: S1074-7613(21)00001-7. doi: 10.1016/j.immuni.2021.01.001. PubMed Article
Dubrot J, Lane-Reticker SK, Kessler EA, Ayer A, Mishra G, Wolfe CH, Zimmer MD, Du PP, Mahapatra A, Ockerman KM, Davis TGR, Kohnle IC, Pope HW, Allen PM, Olander KE, Iracheta-Vellve A, Doench JG, Haining WN, Yates KB, Manguso RT
Immunity. 2021 Jan 20. pii: S1074-7613(21)00001-7. doi: 10.1016/j.immuni.2021.01.001. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 162070 | pSCAR_sgRNA_hygro-tagBFP-lox5171 | 3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistance |
| 162071 | pSCAR_sgRNA_blast-tagBFP-lox2272 | 3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and blasticidin resistance |
| 162072 | pSCAR_sgRNA_blast-tagBFP-lox5171 | 3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and blasticidin resistance |
| 162073 | pLX_EFS-Cre_ppt-del | Compact Cre expression vector with modified PPT designed to generate high-titer non-integrating lentivirus when packaged with PsPax2-D64V (Addgene #63586) |
| 162074 | pSCAR_Cas9-blast_GFP | 3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistance |
| 162075 | pSCAR_Cas9-hygro_GFP | 3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and hygro resistance |
| 162076 | pSCAR_sgRNA_puro-mKate-lox2272 | 3rd generation lentivirus sgRNA delivery backbone with Cre-removable mKate2 reporter and puromycin resistance |
| 162077 | pSCAR_sgRNA_puro-mKate-lox5171 | 3rd generation lentivirus sgRNA delivery backbone with Cre-removable mKate2 reporter and puromycin resistance |
| 162078 | pSCAR_sgRNA_hygro-tagBFP-lox2272 | 3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistance |