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Natural variants suppress mutations in hundreds of essential genes.
Parts L, Batte A, Lopes M, Yuen MW, Laver M, San Luis BJ, Yue JX, Pons C, Eray E, Aloy P, Liti G, van Leeuwen J
Mol Syst Biol. 2021 May;17(5):e10138. doi: 10.15252/msb.202010138.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
162040pML104-kanR1136Expresses Cas9 and contains kanR1136 guide which targets the kanR gene
162041pML107-kanR468Expresses Cas9 and contains kanR468 guide which targets the kanR gene
162042pML104-natR412Expresses Cas9 and contains natR412 guide which targets the natR gene
162043pML107-natR854Expresses Cas9 and contains natR854 guide which targets the natR gene

Antibodies from Article