An enhanced yeast display platform demonstrates the binding plasticity under various selection pressures
Zahradník J, Dey D, Marciano S, Schreiber G
bioRxiv Preprint 2020 Article
Zahradník J, Dey D, Marciano S, Schreiber G
bioRxiv Preprint 2020 Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 162450 | pJYDN | Yeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin and eUnaG2 bilirubin dependent green-yellow fluorescent protein for detection of expression. |
| 162451 | pJYDNp | Positive control plasmid carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid serves as an expression control. |
| 162452 | pJYDNg | Yeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin. The plasmid is carrying the eUnaG2 fluorescent protein and long 2G linker. |
| 162453 | pJYDNgp | Positive control plasmid for pJYDNg carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid contains a long 2G linker. |
| 162454 | pJYDN2 | Yeast display plasmid intended for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA) and expression of N-terminal fusions with Aga2p yeast agglutinin. |
| 162455 | pJYDN2p | Positive control plasmid for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA). |
| 162456 | pJYDN3 | Yeast display platform plasmid N3 which allows for simultaneous expression of the protein of interest at the N-terminus of Aga2p yeast agglutinin and eUnaG2 or nbALFA detection. |
| 162457 | pJYDN3p | Positive control plasmid for pJYDN3n plasmid expression attempts. It contains both eUnaG2 bilirubin dependent fluorescent protein and ALFA-tag for DnbALFA based detection on the cell surface. |
| 162458 | pJYDC1 | Yeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter, eUnaG2 bilirubin dependent green-yellow fluorescent protein, is fused to the Nterminus |
| 162459 | pJYDC2 | Yeast display plasmid for expression of C-terminal fusions. Modification of pJYDC1 vector with ALFA tag in MCS. |
| 162460 | pJYDC3 | Yeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter DnbALFA was fused to the N-terminus of Aga2p. |