Universal Single-Residue Terminal Labels for Fluorescent Live Cell Imaging of Microproteins.
Lafranchi L, Schlesinger D, Kimler KJ, Elsasser SJ
J Am Chem Soc. 2020 Nov 25;142(47):20080-20087. doi: 10.1021/jacs.0c09574. Epub 2020 Nov 11. PubMed Article
Lafranchi L, Schlesinger D, Kimler KJ, Elsasser SJ
J Am Chem Soc. 2020 Nov 25;142(47):20080-20087. doi: 10.1021/jacs.0c09574. Epub 2020 Nov 11. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 162801 | pAS1_4x7SKPylT_EF1_Ub-BsmBI | STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expression |
| 162802 | pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA | STELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient expression |
| 162803 | pAS1_4x7SKPylT_EF1_Ub(G75,76A)-BsmBI | Control STELLA destination plasmid for N-terminally labelled proteins |
| 162804 | pAS1_4x7SKPylT_EF1_Ub-BsmBI_IRES_BSD | STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration |
| 162805 | pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA_IRES_BSD | STELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration |
| 162806 | pAS1_4x7SKPylT_EF1_Ub-*GFP | STELLA plasmid for amber suppression of N-terminally labelled GFP |
| 162807 | pAS1_4x7SKPylT_EF1_Ub-*NoBody | STELLA plasmid for amber suppression of N-terminally labelled NoBody |
| 162808 | pAS1_4x7SKPylT_EF1_Ub-*SMIM38 | STELLA plasmid for amber suppression of N-terminally labelled SMIM38 |
| 162809 | pAS_4x7SKPylT_EF1_Ub-*PIGBOS | STELLA plasmid for amber suppression of N-terminally labelled PIGBOS |
| 162810 | pAS1_4xh7SKPylT_EF1_BsmBI_Intein_DD | STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expression |
| 162811 | pAS1_4xh7SKPylT_EF1_BsmBI_(C1A)-Intein_DD | Control STELLA destination plasmid for C-terminal labelled proteins. Contains restriction sites for golden gate cloning. |
| 162812 | pAS1_4xh7SKPylT_EF1_GFP*_Intein_DD | STELLA plasmid for amber suppression of C-terminally labelled GFP |
| 162813 | pAS1_4xh7SKPylT_EF1_NoBody*_Intein_DD | STELLA plasmid for amber suppression of C-terminally labelled NoBody |
| 162814 | pAS1_4xh7SKPylT_EF1_SMIM38*_Intein_DD | STELLA plasmid for amber suppression of C-terminally labelled SMIM38 |
| 162815 | pAS1_4x7SKPylT_EF1_Ub-*CoV2_M | STELLA plasmid for amber suppression of N-terminally labelled CoV2_M |
| 162816 | pAS1_4x7SKPylT_EF1_Ub-*CoV2_9b | STELLA plasmid for amber suppression of N-terminally labelled CoV2_9b |
| 162817 | pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF6 | STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF6 |
| 162818 | pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF7b | STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF7b |
| 162819 | pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF10 | STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF10 |
| 162820 | pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF9c/14 | STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF9c/14 |
| 162821 | pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b_alt | STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b_alt |
| 162822 | pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b(CoV1h) | STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b(CoV1h) |