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Universal Single-Residue Terminal Labels for Fluorescent Live Cell Imaging of Microproteins.
Lafranchi L, Schlesinger D, Kimler KJ, Elsasser SJ
J Am Chem Soc. 2020 Nov 25;142(47):20080-20087. doi: 10.1021/jacs.0c09574. Epub 2020 Nov 11. PubMed Article

Plasmids from Article

ID Plasmid Purpose  
162801pAS1_4x7SKPylT_EF1_Ub-BsmBISTELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expression
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162802pAS1_4x7SKPylT_EF1_Ub-BsmBI-HASTELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient expression
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162803pAS1_4x7SKPylT_EF1_Ub(G75,76A)-BsmBI Control STELLA destination plasmid for N-terminally labelled proteins
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162806pAS1_4x7SKPylT_EF1_Ub-*GFPSTELLA plasmid for amber suppression of N-terminally labelled GFP
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162807pAS1_4x7SKPylT_EF1_Ub-*NoBodySTELLA plasmid for amber suppression of N-terminally labelled NoBody
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162808pAS1_4x7SKPylT_EF1_Ub-*SMIM38STELLA plasmid for amber suppression of N-terminally labelled SMIM38
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162809pAS_4x7SKPylT_EF1_Ub-*PIGBOSSTELLA plasmid for amber suppression of N-terminally labelled PIGBOS
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162810pAS1_4xh7SKPylT_EF1_BsmBI_Intein_DD STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expression
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162811pAS1_4xh7SKPylT_EF1_BsmBI_(C1A)-Intein_DD Control STELLA destination plasmid for C-terminal labelled proteins. Contains restriction sites for golden gate cloning.
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162812pAS1_4xh7SKPylT_EF1_GFP*_Intein_DD STELLA plasmid for amber suppression of C-terminally labelled GFP
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162813pAS1_4xh7SKPylT_EF1_NoBody*_Intein_DDSTELLA plasmid for amber suppression of C-terminally labelled NoBody
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162814pAS1_4xh7SKPylT_EF1_SMIM38*_Intein_DDSTELLA plasmid for amber suppression of C-terminally labelled SMIM38
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162815pAS1_4x7SKPylT_EF1_Ub-*CoV2_M STELLA plasmid for amber suppression of N-terminally labelled CoV2_M
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162816pAS1_4x7SKPylT_EF1_Ub-*CoV2_9b STELLA plasmid for amber suppression of N-terminally labelled CoV2_9b
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162817pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF6STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF6
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162818pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF7bSTELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF7b
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162819pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF10STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF10
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162820pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF9c/14 STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF9c/14
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162821pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b_alt STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b_alt
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162822pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b(CoV1h) STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b(CoV1h)
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