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Genetic Incorporation of Two Mutually Orthogonal Bioorthogonal Amino Acids That Enable Efficient Protein Dual-Labeling in Cells.
Bednar RM, Jana S, Kuppa S, Franklin R, Beckman J, Antony E, Cooley RB, Mehl RA
ACS Chem Biol. 2021 Nov 19;16(11):2612-2622. doi: 10.1021/acschembio.1c00649. Epub 2021 Sep 30. PubMed Article

Plasmids from Article

ID Plasmid Purpose
164579pEVOL-pAzF[TAG]Machinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codons
164580pUltraI-Tet3.0[TAA]Machinery plasmid containing a single copy of the Mb Tet3.0RS and cognate tRNA for incorporation of Tet3.0 at TAA codons
164581pET28-sfGFP[N150TAA]superfolder GFP with N150TAA mutation
164582pET28-sfGFP[D134TAG+N150TAA]superfolder GFP with D134TAG and N150TAA mutations

Antibodies from Article