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Optogenetic control of apical constriction induces synthetic morphogenesis in mammalian tissues.
Martinez-Ara G, Taberner N, Takayama M, Sandaltzopoulou E, Villava CE, Bosch-Padros M, Takata N, Trepat X, Eiraku M, Ebisuya M
Nat Commun. 2022 Sep 14;13(1):5400. doi: 10.1038/s41467-022-33115-0. PubMed Article

Plasmids from Article

ID Plasmid Purpose
170976pDONRp5-p2 GFP-NShroom3-iLIDN-terminal component of OptoShroom3 optogenetic tool. Binds to apical junctions. pDONRp5-p2 plasmid.
170977pDONRp5-p2 SspB-mCherry-CShroom3C-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.

Antibodies from Article