Anterograde transneuronal tracing and genetic control with engineered yellow fever vaccine YFV-17D.
Li E, Guo J, Oh SJ, Luo Y, Oliveros HC, Du W, Arano R, Kim Y, Chen YT, Eitson J, Lin DT, Li Y, Roberts T, Schoggins JW, Xu W
Nat Methods. 2021 Nov 25. pii: 10.1038/s41592-021-01319-9. doi: 10.1038/s41592-021-01319-9. PubMed Article
Li E, Guo J, Oh SJ, Luo Y, Oliveros HC, Du W, Arano R, Kim Y, Chen YT, Eitson J, Lin DT, Li Y, Roberts T, Schoggins JW, Xu W
Nat Methods. 2021 Nov 25. pii: 10.1038/s41592-021-01319-9. doi: 10.1038/s41592-021-01319-9. PubMed Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
175273 | pAAV-DIO-NS1 | AAV vector mediating Cre-depenent expression of NS1 gene of YFV-17D |
175274 | pAAV-rtTA | AAV vector mediating neuron-specific expression of rtTA |
175275 | pAAV-SynaptoTAG2 | AAV vector for mapping synaptic projections of infected neurons; expresses tdTomato to reveal neuronal soma and axons, and expresses EGFP fused to Synaptobrevin-2 to track synaptic terminals. |
175276 | AAV-Syn-NS1 | AAV vector mediating bicistronic expression of NS1 gene of YFV-17D and dTomato with NLS |
175277 | AAV-TRE-C-prM-E-NS1 | AAV vector mediating inducible expression of 4 genes (the C-prM-E-NS1) of YFV-17D |
175278 | AAV-TRE-NS1-dTomato | AAV vector mediating inducible bicistronic expression of NS1 gene of YFV-17D and dTomato |
175279 | AAV-TRE-NS1NF | AAV vector mediating inducible expression of the NS1 gene of YFV-17D |
175280 | AAV-tTA | AAV vector mediating neuron-specific expression of tTA |
175281 | FUW-C-prM-E-NS1 | lentiviral vector mediating bicistronic expression of the 4 genes of YFV-17D (C-prM-E-NS1) and EGFP. |
179952 | YFVdelNS1-Cre | pCCI vector encoding YFV-17D lacking nonstructural protein 1 (NS1) but with Cre |
179953 | YFVdelCME-mVenus | pCCI vector encoding YFV-17D lacking C, prM, E proteins but with mVenus |