NMDA Receptors in the Lateral Preoptic Hypothalamus Are Essential for Sustaining NREM and REM Sleep.
Miracca G, Anuncibay-Soto B, Tossell K, Yustos R, Vyssotski AL, Franks NP, Wisden W
J Neurosci. 2022 May 31. pii: JNEUROSCI.0350-21.2022. doi: 10.1523/JNEUROSCI.0350-21.2022. PubMed Article
Miracca G, Anuncibay-Soto B, Tossell K, Yustos R, Vyssotski AL, Franks NP, Wisden W
J Neurosci. 2022 May 31. pii: JNEUROSCI.0350-21.2022. doi: 10.1523/JNEUROSCI.0350-21.2022. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 182246 | pAAV-iCre-2A-cherry | Expresses iCre linked to mCherry. The promoter is a fragment of the hdc gene promoter which drives pan neuronal expression. |
| 182499 | pAAV-iCre-2A-venus | Expresses iCre-venus. The promoter is a fragment of the hdc gene promoter that gives pan neuronal expression |
| 182502 | pAAV-flex-GFP-shRNA-GluN1 | Expresses shRNA against mouse NMDA receptor NR1 subunit. Flexed cassette driven by hdc (pan neuronal) gene promoter |
| 182503 | pAAV-flex-GFP-shRNA-scramble | Expresses scramble shRNA for control expts. Flexed cassette driven by pan neuronal gene promoter |
| 184331 | pAAV-HDC-DIO-GFP-shRNA.scramble | Expresses scramble shRNA for control expts. Flexed (DIO) cassette driven by hdc promoter fragment for pan neuronal expression |