Addgene: Two-stage dynamic deregulation of metabolism improves process robustness & scalability in engineered E. coli.

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Two-stage dynamic deregulation of metabolism improves process robustness & scalability in engineered E. coli.
Ye Z, Li S, Hennigan JN, Lebeau J, Moreb EA, Wolf J, Lynch MD
Metab Eng. 2021 Nov;68:106-118. doi: 10.1016/j.ymben.2021.09.009. Epub 2021 Sep 30. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
71326	pSMART-Ala2	NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression
71348	pCASCADE-gltA1-gltA2	gltA1-gltA2 targeting guide RNA E. coli , Low Phosphate Induction
87172	pSMART-ala11	NADPH dependent Alanine Dehydrogenase, Constitutive E. coli Expression
87173	pSMART-ala12	NADPH dependent Alanine Dehydrogenase, Constitutive E. coli Expression
87174	pSMART-ala13	NADPH dependent Alanine Dehydrogenase, Constitutive E. coli Expression

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