pCASCADE-gltA1-gltA2
(Plasmid
#71348)
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PurposegltA1-gltA2 targeting guide RNA E. coli , Low Phosphate Induction
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71348 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCASCADE
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Backbone manufacturerNone - Constructed in House
- Backbone size w/o insert (bp) 2500
- Total vector size (bp) 2905
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namegltA1-gltA2 guide array
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gRNA/shRNA sequence3 Guide Array : E . coli gltA1 and gltA2 gene promoters
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SpeciesSynthetic
- Promoter E . coli ugpB gene promoter, low phosphate induction
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer GGGAGACCACAACGG
- 3′ sequencing primer AGTCCAGCTTGGAGCG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Read the preprint at bioRxiv: https://www.biorxiv.org/content/10.1101/2020.08.30.274290v1.full.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCASCADE-gltA1-gltA2 was a gift from Michael Lynch (Addgene plasmid # 71348 ; http://n2t.net/addgene:71348 ; RRID:Addgene_71348) -
For your References section:
Two-stage dynamic deregulation of metabolism improves process robustness & scalability in engineered E. coli. Ye Z, Li S, Hennigan JN, Lebeau J, Moreb EA, Wolf J, Lynch MD. Metab Eng. 2021 Nov;68:106-118. doi: 10.1016/j.ymben.2021.09.009. Epub 2021 Sep 30. 10.1016/j.ymben.2021.09.009 PubMed 34600151
