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Development of an Haa1-based biosensor for acetic acid sensing in Saccharomyces cerevisiae.
Mormino M, Siewers V, Nygard Y
FEMS Yeast Res. 2021 Sep 11;21(6). pii: 6363685. doi: 10.1093/femsyr/foab049.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
183057pMM2_4HAA1-mTurquoise2 under HAA1p and mRuby2 under YGP1p with flanks for genome integration into HO locus
183058pMM2_9Biosensor for acetic acid: BM3R1-Haa1-mTurquoise2 under HAA1p and mCherry under BM3R1ENO1cp with flanks for genome integration in toHO locus
183059pMM2_10Control for biosensor in pMM2_9: HAA1-mTurquoise2 under HAA1p and mCherry under HREsYGP1p with flanks for genome integration into HO locus
183060pMM2_11Control for biosensor in pMM2_9: HAA1-mTurquoise2 under HAA1p and mCherry under HREsENO1cp with flanks for genome integration into HO locus
183061pMM2_12Control for biosensor in pMM2_9: BM3R1-HAA1-mTurquoise2 under HAA1p and mCherry under HREsYGP1p with flanks for genome integration into HO locus

Antibodies from Article