pMM2_10
(Plasmid #183059)

• Purpose: Control for biosensor in pMM2_9: HAA1-mTurquoise2 under HAA1p and mCherry under HREsYGP1p with flanks for genome integration into HO locus
• Depositing Lab: Yvonne Nygård
• Publication: Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii: 6363685. doi: 10.1093/femsyr/foab049.

Backbone

• Vector backbone: MoClo-YTK
• Total vector size (bp): 9866
• Vector type: Yeast Expression, Synthetic Biology
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: mCherry
• Species: Synthetic
• Promoter: Synthetic promoter
• Tag / Fusion Protein:
  • mCherry

Cloning Information for Gene/Insert 1

• Cloning method: Gateway Cloning
• 5′ sequencing primer: Golden Gate Assembly

Gene/Insert 2

• Gene/Insert name: HAA1-mTurquoise2
• Species: S. cerevisiae (budding yeast), Synthetic
• Promoter: HAA1
• Tag / Fusion Protein:
  • mTurquoise2

Cloning Information for Gene/Insert 2

• Cloning method: Gateway Cloning
• 5′ sequencing primer: Golden Gate Assembly

Resource Information

• Supplemental Documents:
  • MM2_10 - Haa1-CFP_YGP1-HREs-mCherry.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid parts were synthesized or taken from the Yeast MoClo kit.