Kinase-independent synthesis of 3-phosphorylated phosphoinositides by a phosphotransferase.
Walpole GFW, Pacheco J, Chauhan N, Clark J, Anderson KE, Abbas YM, Brabant-Kirwan D, Montano-Rendon F, Liu Z, Zhu H, Brumell JH, Deiters A, Stephens LR, Hawkins PT, Hammond GRV, Grinstein S, Fairn GD
Nat Cell Biol. 2022 Apr 28. pii: 10.1038/s41556-022-00895-y. doi: 10.1038/s41556-022-00895-y. PubMed Article
Walpole GFW, Pacheco J, Chauhan N, Clark J, Anderson KE, Abbas YM, Brabant-Kirwan D, Montano-Rendon F, Liu Z, Zhu H, Brumell JH, Deiters A, Stephens LR, Hawkins PT, Hammond GRV, Grinstein S, Fairn GD
Nat Cell Biol. 2022 Apr 28. pii: 10.1038/s41556-022-00895-y. doi: 10.1038/s41556-022-00895-y. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 183651 | NES-EGFP-PH-BTKx1 | PtdIns(3,4,5)P3 biosensor |
| 183652 | NES-EGFP-PH-BTKx2 | PtdIns(3,4,5)P3 biosensor |
| 183653 | NES-mCherry-PH-BTKx1 | PtdIns(3,4,5)P3 biosensor |
| 183654 | NES-mCherry-PH-BTKx2 | PtdIns(3,4,5)P3 biosensor |
| 183655 | SopB-EGFP | C-terminally tagged Salmonella Typhimurium SopB for mammalian expression |
| 183656 | SopB(C460S)-EGFP | C-terminally tagged Salmonella Typhimurium SopB (catalytically inactive) for mammalian expression |
| 183657 | EGFP-SopB | N-terminally tagged Salmonella Typhimurium SopB for mammalian expression |
| 183658 | EGFP-SopB(C460S) | N-terminally tagged Salmonella Typhimurium SopB (catalytically inactive) for mammalian expression |
| 183659 | EGFP-SopB(K525A) | N-terminally tagged Salmonella Typhimurium SopB (K525A) for mammalian expression |
| 183660 | EGFP-SopB(K528A) | N-terminally tagged Salmonella Typhimurium SopB (K528A) for mammalian expression |
| 183661 | EGFP-SopB(33-554) | N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression |
| 183662 | EGFP-SopB(33-520) | N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression |
| 183663 | EGFP-SopB(68-554) | N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression |
| 183664 | EGFP-SopB(172-554) | N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression |
| 183665 | EGFP-SopB(211-554) | N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression |
| 183666 | EGFP-IpgD | N-terminally tagged Shigella flexneri IpgD for mammalian expression |
| 183667 | EGFP-IpgD(C439S) | N-terminally tagged Shigella flexneri IpgD (catalytically inactive) for mammalian expression |
| 183668 | pUG36-NES-EGFP-cPHx3 | PtdIns(3,4)P2 biosensor for expression in yeast |
| 183669 | pUG34-NES-EGFP-cPHx3 | PtdIns(3,4)P2 biosensor for expression in yeast |
| 183670 | pESC-LEU-SopB | Inducible Salmonella Typhimurium SopB for expression in yeast |
| 183671 | pESC-Leu-SopB(C460S) | Inducible Salmonella Typhimurium SopB (catalytically inactive) for expression in yeast |
| 183672 | pESC-LEU-IpgD | Inducible Shigella flexneri IpgD for expression in yeast |
| 183673 | pESC-LEU-IpgD(C439S) | Inducible Shigella flexneri IpgD (catalytically inactive) for expression in yeast |
| 183674 | His6-EGFP-cPHx1 | PtdIns(3,4)P2 biosensor for purification from bacteria |
| 183675 | His6-EGFP-PH-PLCD1 | PtdIns(4,5)P2 biosensor for purification from bacteria |
| 183676 | His6-SMT3-SopB | Recombinant Salmonella Typhimurium SopB |
| 183677 | His6-SMT3-SopB(C460S) | Recombinant Salmonella Typhimurium SopB (catalytically inactive) |
| 183678 | mRFP-FKBP-INPP5E(D556A) | Recruitable human type IV 5-phosphatase enzyme (catalytically inactive) |