Anti-CRISPR prediction using deep learning reveals an inhibitor of Cas13b nucleases.
Wandera KG, Alkhnbashi OS, Bassett HVI, Mitrofanov A, Hauns S, Migur A, Backofen R, Beisel CL
Mol Cell. 2022 May 24. pii: S1097-2765(22)00437-3. doi: 10.1016/j.molcel.2022.05.003. PubMed Article
Wandera KG, Alkhnbashi OS, Bassett HVI, Mitrofanov A, Hauns S, Migur A, Backofen R, Beisel CL
Mol Cell. 2022 May 24. pii: S1097-2765(22)00437-3. doi: 10.1016/j.molcel.2022.05.003. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 184565 | pPbuCas13b | Inducible expression of PbuCas13b nuclease in bacteria. |
| 184566 | pAcrVIB1 | Inducible expression of anti-CRISPR protein AcrVIB1 in bacteria. |
| 184567 | pPbuCas13b-gRNA-1 | Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria. |
| 184568 | pPbuCas13b-gRNA-NT | Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PbuCas13b in bacteria. |
| 184833 | pPgiCas13b | Inducible expression of PgiCas13b nuclease in bacteria. |
| 184834 | pPgiCas13b-gRNA-1 | Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria. |
| 184835 | pPgiCas13b-gRNA-NT | Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PgiCas13b in bacteria. |
| 184836 | pAcrVIB1-cloDF-kan | Expression of anti-CRISPR protein AcrVIB1 in bacteria. |
| 184837 | pcloDF-kan | Backbone that confers Kanamycin resistance, contains cloDF ori |
| 184838 | pPlacPbuCas13b-gRNA-NT | Expression of single-spacer CRISPR array with a shortened non-targeting spacer for PbuCas13b and expression of PbuCas13b in bacteria. |
| 184839 | pPlacPbuCas13b-gRNA-T-MS2 | Expression of a single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome and expression of PbuCas13b in bacteria. |
| 184840 | pPlacPbuCas13b-gRNA-T-deGFP | Expression of a single-spacer CRISPR array with spacer #1 targeting the mRNA of deGFP and expression of PbuCas13b in bacteria. |
| 184841 | P70a-deGFP-sc101-kan | Expression of deGFP |
| 184842 | pPJ23116-MS2-rep | Expression of the rep protein of phage MS2 |
| 184843 | pAcrVIB1-cloDF-amp | Expression of anti-CRISPR protein AcrVIB1 in bacteria. |
| 184844 | pcloDF-amp | Backbone that confers Ampicillin resistance, contains cloDF ori |