Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Friction-driven membrane scission by the human ESCRT-III proteins CHMP1B and IST1.
Cada AK, Pavlin MR, Castillo JP, Tong AB, Larsen KP, Ren X, Yokom AL, Tsai FC, Shiah JV, Bassereau PM, Bustamante CJ, Hurley JH
Proc Natl Acad Sci U S A. 2022 Jul 19;119(29):e2204536119. doi: 10.1073/pnas.2204536119. Epub 2022 Jul 11.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
184803His-MBP-CHMP1B, S2CBacterial expression of CHMP1B (S2C mutation)
184804His-GST-Cys-M87 SpastinBacterial expression of M87 Spastin (A2C mutation)
184805His-MBP-IST1 N-terminal domainBacterial expression of IST1 N-terminal domain
184806VPS4B, S2CBacterial expression of VPS4B (S2C mutation)

Antibodies from Article