Genome editing in animals with minimal PAM CRISPR-Cas9 enzymes.
Vicencio J, Sanchez-Bolanos C, Moreno-Sanchez I, Brena D, Vejnar CE, Kukhtar D, Ruiz-Lopez M, Cots-Ponjoan M, Rubio A, Melero NR, Crespo-Cuadrado J, Carolis C, Perez-Pulido AJ, Giraldez AJ, Kleinstiver BP, Ceron J, Moreno-Mateos MA
Nat Commun. 2022 May 12;13(1):2601. doi: 10.1038/s41467-022-30228-4. PubMed Article
Vicencio J, Sanchez-Bolanos C, Moreno-Sanchez I, Brena D, Vejnar CE, Kukhtar D, Ruiz-Lopez M, Cots-Ponjoan M, Rubio A, Melero NR, Crespo-Cuadrado J, Carolis C, Perez-Pulido AJ, Giraldez AJ, Kleinstiver BP, Ceron J, Moreno-Mateos MA
Nat Commun. 2022 May 12;13(1):2601. doi: 10.1038/s41467-022-30228-4. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 179316 | pT3TS-zSpG | In vitro transcription of SpG-Cas9 mRNA zebrafish codon-optimized from T3 promoter |
| 179317 | pT3TS-zSpRY | In vitro transcription of SpRY-Cas9 mRNA zebrafish codon-optimized from T3 promoter |
| 179318 | pET28b-SpG-His | Plasmid for bacterial expression and purification of SpG-Cas9 |
| 179320 | pET28b-SpRY-His | Plasmid for bacterial expression and purification of SpRY-Cas9 |