pT3TS-zSpRY
(Plasmid #179317)

• Purpose: In vitro transcription of SpRY-Cas9 mRNA zebrafish codon-optimized from T3 promoter
• Depositing Lab: Miguel Angel Moreno-Mateos
• Publication: Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi: 10.1038/s41467-022-30228-4.

Backbone

• Vector backbone: pT3TS
• Backbone size w/o insert (bp): 3150
• Total vector size (bp): 7330
• Vector type: CRISPR ; In vitro transcription by T3 promoter

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Zebrafish codon-optimized Cas9 variant SpRY
• Alt name: SpRY
• Species: Synthetic
• Insert Size (bp): 4104
• Mutation: SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N1317R/A1322R/R1333P/R1335Q/T1337R
• Tags / Fusion Proteins:
  • SV40-NLS (N terminal on insert)
  • SV40-NLS (C terminal on insert)

Resource Information

• Supplemental Documents:
  • pT3TS-SpRY.gbk
• A portion of this plasmid was derived from a plasmid made by: pT3TS_zCas9 (Addgene; 46757) (Jao et al., 2013) was modified by site-directed mutagenesis to generate this plasmid encoding the SpRY zebrafish codon-optimized variant

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is owned by Moreno-Mateos Lab (CABD, Seville, Spain).

Please visit https://www.biorxiv.org/content/10.1101/2021.06.06.447255v2 for bioRxiv preprint.