pYtags enable spatiotemporal measurements of receptor tyrosine kinase signaling in living cells.
Farahani PE, Yang X, Mesev EV, Fomby KA, Brumbaugh-Reed EH, Bashor CJ, Nelson CM, Toettcher JE
Elife. 2023 May 22;12:e82863. doi: 10.7554/eLife.82863. PubMed Article
Farahani PE, Yang X, Mesev EV, Fomby KA, Brumbaugh-Reed EH, Bashor CJ, Nelson CM, Toettcher JE
Elife. 2023 May 22;12:e82863. doi: 10.7554/eLife.82863. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 188626 | pHR EGFR-CD3ε-FusionRed | Human EGFR labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensor |
| 188627 | pHR iRFP-ZtSH2 | Fluorescent reporter for CD3ε/ZtSH2 pYtag biosensor |
| 188628 | pHR ErbB2-CD3ε-FusionRed | Human ErbB2 labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensor |
| 188629 | pHR Clover-VISH2 | Fluorescent reporter for SLP76/VISH2 pYtag biosensor |
| 188630 | pHR EGFR-SLP76-TagBFP | Human EGFR labeled with SLP76 for monitoring with SLP76/VISH2 pYtag biosensor |
| 188631 | pHR Grb2-TagBFP | Human Grb2 C-terminally labeled with TagBFP |
| 188632 | pHR FGFR1-CD3ε-FusionRed | Human FGFR1 labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensor |
| 188633 | pX330 EGFR-sgRNA | A human codon-optimized SpCas9 and chimeric guide RNA targeting C-terminus of human EGFR |
| 188634 | pUC19 EGFRup-CD3ε-mNeonGreen-EGFRdown | HDR template for CD3ε-mNeonGreen knock-in at C-terminus of human EGFR |