Designed active-site library reveals thousands of functional GFP variants.
Weinstein JY, Marti-Gomez C, Lipsh-Sokolik R, Hoch SY, Liebermann D, Nevo R, Weissman H, Petrovich-Kopitman E, Margulies D, Ivankov D, McCandlish DM, Fleishman SJ
Nat Commun. 2023 May 20;14(1):2890. doi: 10.1038/s41467-023-38099-z. PubMed Article
Weinstein JY, Marti-Gomez C, Lipsh-Sokolik R, Hoch SY, Liebermann D, Nevo R, Weissman H, Petrovich-Kopitman E, Margulies D, Ivankov D, McCandlish DM, Fleishman SJ
Nat Commun. 2023 May 20;14(1):2890. doi: 10.1038/s41467-023-38099-z. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 191877 | pET28 slow.3 | Encodes a GFP design, slow.3 |
| 191878 | pET28 bleach.2 | Encodes a GFP design, bleach.2 |
| 191879 | pET28 bleach.6 | Encodes a GFP design, bleach.6 |
| 191880 | pET28 mid.2 | Encodes a GFP design, mid.2 |
| 191881 | pET28 basic.3 | Encodes a GFP design, basic.3 |
| 191882 | pET28 thermo.9 | Encodes a GFP design, thermo.9 |
| 191884 | pET28 mid.3 | Encodes a GFP design, mid.3 |
| 191885 | pET28 bleach.1 | Encodes a GFP design, bleach.1 |
| 191894 | pET28 slow.2 | Encodes a GFP design, slow.2 |
| 191895 | pET28 mid.4 | Encodes a GFP design, mid.4 |
| 191896 | pET28 mid.5 | Encodes a GFP design, mid.5 |
| 191897 | pET28 acid.3 | Encodes a GFP design, acid.3 |
| 191898 | pET28 acid.4 | Encodes a GFP design, acid.4 |
| 191899 | pET28 mid.6 | Encodes a GFP design, mid.6 |
| 191900 | pET28 mid.7 | Encodes a GFP design, mid.7 |
| 191901 | pET28 em-blue-shift.2 | Encodes a GFP design, em-blue-shift.2 |
| 191902 | pET28 mid.8 | Encodes a GFP design, mid.8 |
| 191903 | pET28 acid.5 | Encodes a GFP design, acid.5 |
| 191911 | pET28 basic.2 | Encodes a GFP design, basic.2 |
| 191912 | pET28 acid.6 | Encodes a GFP design, acid.6 |
| 191913 | pET28 mid.12 | Encodes a GFP design, mid.12 |
| 191914 | pET28 bleach.3 | Encodes a GFP design, bleach.3 |
| 191915 | pET28 mid.13 | Encodes a GFP design, mid.13 |
| 191919 | pET28 sf:em-blue-shift.2 | Encodes a GFP design, sf:em-blue-shift.2 |
| 191921 | pET28 sf:mid.11 | Encodes a GFP design, sf:mid.11 |
| 191925 | pET28 sf:bleach.3 | Encodes a GFP design, sf:bleach.3 |
| 191926 | pET28 sf:acid.3 | Encodes a GFP design, sf:acid.3 |
| 191928 | pET28 sf:em-blue-shift.1 | Encodes a GFP design, sf:em-blue-shift.1 |
| 191929 | pET28 sf:bleach.1 | Encodes a GFP design, sf:bleach.1 |
| 191933 | pET28 sf:mid.7 | Encodes a GFP design, sf:mid.7 |
| 191934 | pET28 sf:mid.10 | Encodes a GFP design, sf:mid.10 |