An AAV-CRISPR/Cas9 strategy for gene editing across divergent rodent species: Targeting neural oxytocin receptors as a proof of concept.
Boender AJ, Boon M, Albers HE, Eck SR, Fricker BA, Kelly AM, LeDoux JE, Motta SC, Shrestha P, Taylor JH, Trainor BC, Triana-Del Rio R, Young LJ
Sci Adv. 2023 Jun 2;9(22):eadf4950. doi: 10.1126/sciadv.adf4950. Epub 2023 May 31. PubMed Article
Boender AJ, Boon M, Albers HE, Eck SR, Fricker BA, Kelly AM, LeDoux JE, Motta SC, Shrestha P, Taylor JH, Trainor BC, Triana-Del Rio R, Young LJ
Sci Adv. 2023 Jun 2;9(22):eadf4950. doi: 10.1126/sciadv.adf4950. Epub 2023 May 31. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 194015 | pAAV-U6-sgRNA(OXTR.1)-CMV-eGFP | Expresses a gRNA that targets OXTR coding sequence in multiple (rodent) species and eGFP |
| 194016 | pAAV-U6-sgRNA(OXTR.2)-CMV-eGFP | Expresses a gRNA that targets OXTR coding sequence in multiple (rodent) species and eGFP |
| 194017 | pAAV-U6-sgRNA(CTRL)-CMV-eGFP | Expresses a gRNA that targets the LacZ gene (serves as control) and eGFP |