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Comprehensive Profiling of Four Base Overhang Ligation Fidelity by T4 DNA Ligase and Application to DNA Assembly.
Potapov V, Ong JL, Kucera RB, Langhorst BW, Bilotti K, Pryor JM, Cantor EJ, Canton B, Knight TF, Evans TC Jr, Lohman GJS
ACS Synth Biol. 2018 Nov 16;7(11):2665-2674. doi: 10.1021/acssynbio.8b00333. Epub 2018 Oct 29. PubMed Article

These materials are included in the High Complexity Golden Gate Assembly Standards Kit (24 fragment lac cassette).

Plasmids from Article

ID Plasmid Purpose
195714pGGAselectpGGAselect is a cloning vector for Golden Gate Assembly using BsaI, BsmBI, and BbsI Type IIS restriction enzymes.
195715pUC57-mini v2-HF24_F1This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
195716pUC57-mini v2-HF24_F2This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
195717pUC57-mini v2-HF24_F3This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
195718pUC57-mini v2-HF24_F4This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
195719pUC57-mini v2-HF24_F5This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
195720pUC57-mini v2-HF24_F6This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
195721pUC57-mini v2-HF24_F7This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
195722pUC57-mini v2-HF24_F8This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
195723pUC57-mini v2-HF24_F9This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
195724pUC57-mini v2-HF24_F10This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
195725pUC57-mini v2-HF24_F11This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
195726pUC57-mini v2-HF24_F12This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
195727pUC57-mini v2-HF24_F13This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
195728pUC57-mini v2-HF24_F14This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
195729pUC57-mini v2-HF24_F15This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
195730pUC57-mini v2-HF24_F16This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
195731pUC57-mini v2-HF24_F17This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
195732pUC57-mini v2-HF24_F18This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
195733pUC57-mini v2-HF24_F19This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
195734pUC57-mini v2-HF24_F20This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
195735pUC57-mini v2-HF24_F21This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
195736pUC57-mini v2-HF24_F22This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
195737pUC57-mini v2-HF24_F23This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
195738pUC57-mini v2-HF24_F24This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Antibodies from Article