VRK1 as a synthetic lethal target in VRK2 promoter-methylated cancers of the nervous system.
So J, Mabe NW, Englinger B, Chow KH, Moyer SM, Yerrum S, Trissal MC, Marques JG, Kwon JJ, Shim B, Pal S, Panditharatna E, Quinn T, Schaefer DA, Jeong D, Mayhew DL, Hwang J, Beroukhim R, Ligon KL, Stegmaier K, Filbin MG, Hahn WC
JCI Insight. 2022 Oct 10;7(19):e158755. doi: 10.1172/jci.insight.158755. PubMed Article
So J, Mabe NW, Englinger B, Chow KH, Moyer SM, Yerrum S, Trissal MC, Marques JG, Kwon JJ, Shim B, Pal S, Panditharatna E, Quinn T, Schaefer DA, Jeong D, Mayhew DL, Hwang J, Beroukhim R, Ligon KL, Stegmaier K, Filbin MG, Hahn WC
JCI Insight. 2022 Oct 10;7(19):e158755. doi: 10.1172/jci.insight.158755. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 199634 | sgCTRL-puro | sgRNA control guide targeting intergenic region |
| 199635 | sgLacZ-puro | sgRNA control guide targeting bacterial LacZ |
| 199636 | sgVRK2-puro | sgRNA guide against VRK2 |
| 199637 | pLenti-SwitchON-sgCh2-2 | Tamoxifen-inducible expression of sgRNA control targeting intergenic region |
| 199638 | pLenti-SwitchON-sgVRK1 | Tamoxifen-inducible expression of sgRNA targeting VRK1 |
| 199639 | sgCTRL-hygro | sgRNA control; induces CAS9 cutting in an intergenic region |
| 199640 | sgVRK1-hygro | sgRNA guide against VRK1 |
| 199641 | pLX317-VRK2 | Expresses VRK2 |
| 199642 | pLX317-VRK2-K168E | Expresses kinase-inactive VRK2 (K168E) |
| 199643 | pLX317-VRK1 | Expresses VRK1 |
| 199644 | pLX317-VRK1-K179E | Expresses kinase-inactive VRK1 (K179E) |
| 199645 | LentiCas9-sgCh2-2-Blast | Expresses Cas9 and sgRNA control guide targeting intergenic region |
| 199646 | LentiCas9-sgVRK1 #1-Blast | Expresses Cas9 and sgRNA guide targeting VRK1 |
| 199647 | LentiCas9-sgVRK1 #4-Blast | Expresses Cas9 and sgRNA guide targeting VRK1 |
| 199648 | BAF-WT-tet-puro | Doxycycline-inducible BAF expression |
| 199649 | BAF-S4A-tet-puro | Doxycycline-inducible BAF expression |
| 199650 | BAF-S4D-tet-puro | Doxycycline-inducible BAF expression |
| 199651 | VRK1-dTag-puro | Expresses VRK1 C-term. Tagged with dTAG |