APOBEC Reporter Systems for Evaluating diNucleotide Editing Levels.
Rieffer AE, Chen Y, Salamango DJ, Moraes SN, Harris RS
CRISPR J. 2023 Oct;6(5):430-446. doi: 10.1089/crispr.2023.0027. Epub 2023 Sep 6. PubMed Article
Rieffer AE, Chen Y, Salamango DJ, Moraes SN, Harris RS
CRISPR J. 2023 Oct;6(5):430-446. doi: 10.1089/crispr.2023.0027. Epub 2023 Sep 6. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 198882 | eGFP Y93H TC editing reporter | Express eGFP fluorescence after Y93H correction and mCherry as transfection control |
| 198883 | eGFP Y93H AC editing reporter | Express eGFP fluorescence after Y93H correction and mCherry as transfection control |
| 198884 | eGFP Y93H GC editing reporter | Express eGFP fluorescence after Y93H correction and mCherry as transfection control |
| 198885 | eGFP Y93H CC editing reporter | Express eGFP fluorescence after Y93H correction and mCherry as transfection control |
| 198886 | A3Bctd-L7G-Cas9n-UGI-NLS | Express A3Bctd-L7G BE3-based base editor |
| 198887 | A3Bctd-D314E-Cas9n-UGI-NLS | Express A3Bctd-D314E BE3-based base editor |
| 198888 | A3Bctd-max | Express A3Bctd-max BE4max-based base editor |
| 198889 | A3B-Cas9n-UGI-NLS | Express full-length A3B BE3-based base editor |
| 198890 | AID∆C-Cas9n-UGI-NLS | Express AID(1-188) BE3-based base editor |
| 198891 | Modular BE4max for GG insertion | Easily insert deaminase of choice into BE4max using Golden Gate cloning |
| 207165 | eA3Ai-Cas9n-UGI-NLS | Express A3A with an N57G point mutation and an intron in a BE3-based base editor |
| 207166 | eA3Ai-max | Express A3A with an N57G point mutation and an intron in a BE4max-based base editor |
| 207167 | A3Bmax | Express full-length human A3B in a BE4max-based base editor |