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Addgene
Optical control of protein activity by fluorescent protein domains.
Zhou XX, Chung HK, Lam AJ, Lin MZ
Science. 2012 Nov 9;338(6108):810-4. doi: 10.1126/science.1226854. PubMed Article

Plasmids from Article

ID Plasmid Purpose
41640pcDNA3-K-I-N-CAAXExpresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to wild-type Dronpa145K at C terminus and Dronpa145N at N terminus, for optogenetic activation
41642pcDNA3-K-I-CAAXCatalytically active control, intersectin Dbl homology (DH) domain fused to wild-type Dronpa
41643pcDNA3-I-K-CAAXCatalytically active control, intersectin Dbl homology (DH) domain fused to wild-type Dronpa
41644pcDNA3-K-CAAXExpresses wild-type Dronpa fused to the membrane-anchoring K-Ras C-terminal farnesylation motif (CAAX box)
41645pcDNA3-mNeptune2-NExpresses far-red FP mNeptune2 fused to Dronpa145N. Dronpa145N oligomerizes under violet light (390-400nm) and dissociates under cyan light (490-500nm)
41686pcDNA3-N-I-N-CAAXExpresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to Dronpa145N at C terminus and at N terminus, for optogenetic activation

Antibodies from Article