RNA-Guided Human Genome Engineering via Cas9.
Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE, Church GM
Science. 2013 Jan 3. PubMed Article
Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE, Church GM
Science. 2013 Jan 3. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 41815 | hCas9 | Expresses human codon optimized Cas9 nuclease for genome engineering |
| 41816 | hCas9_D10A | Expresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineering |
| 41817 | gRNA_AAVS1-T1 | Expresses a guide RNA (gRNA) to target human AAVS1 (T1 target sequence) for genome engineering |
| 41818 | gRNA_AAVS1-T2 | Expresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineering |
| 41819 | gRNA_GFP-T1 | Expresses a guide RNA (gRNA) to target GFP (T1 target sequence) for genome engineering |
| 41820 | gRNA_GFP-T2 | Expresses a guide RNA (gRNA) to target GFP (T2 target sequence) for genome engineering |
| 41821 | gRNA_DNMT3a-T1 | Expresses a guide RNA (gRNA) to target DNMT3a (T1 target sequence) for genome engineering |
| 41822 | gRNA_DNMT3a-T2 | Expresses a guide RNA (gRNA) to target DNMT3a (T2 target sequence) for genome engineering |
| 41823 | gRNA_DNMT3b | Expresses a guide RNA (gRNA) to target DNMT3b for genome engineering |
| 41824 | gRNA_Cloning Vector | An empty gRNA expression vector, used to create a gRNA to a specific sequence. |