Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system.
Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA
Nucleic Acids Res. 2013 Jun 12. PubMed Article
Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA
Nucleic Acids Res. 2013 Jun 12. PubMed Article
Plasmids from Article
ID | Plasmid | Purpose | |
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46569 | pdCas9 | Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers. |
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46570 | pWJ66 | Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9) |
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46571 | pWJ68 | Same as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9) |
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