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pWJ68
(Plasmid #46571)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46571 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    tracrRNA
  • Species
    S. pyogenes

Gene/Insert 2

  • Gene/Insert name
    w-dcas9
  • Species
    S. pyogenes
  • Mutation
    D10A, H840A
  • GenBank ID
    Spy_1046
  • Tag / Fusion Protein
    • rpoZ (N terminal on backbone)

Gene/Insert 3

  • Gene/Insert name
    CRISPR array

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This plasmid was constructed by modifying the pCas9 plasmid constructed in the lab of Feng Zhang at MIT, and published in Nat Biotechnol. 2013 Mar;31(3):233-9. doi: 10.1038/nbt.2508. RNA-guided editing of bacterial genomes using CRISPR-Cas systems
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWJ68 was a gift from Luciano Marraffini (Addgene plasmid # 46571)
  • For your References section:

    Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA. Nucleic Acids Res. 2013 Jun 12. 10.1093/nar/gkt520 PubMed 23761437