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Apical sorting of lysoGPI-anchored proteins occurs independent of association with detergent-resistant membranes but dependent on their N-glycosylation.
Castillon GA, Michon L, Watanabe R
Mol Biol Cell. 2013 Jun;24(12):2021-33. doi: 10.1091/mbc.E13-03-0160. Epub 2013 Apr 24.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
50373pME-hygro-hPGAP3-3HAExpress C-terminally triple HA tagged human PGAP3 in mammmalian cells
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50374pME-puro-SNAP-FLAG-CD59Express SNAP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells
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50375pME-puro-CLIP-FLAG-CD59Express CLIP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells
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50376pCDNA3.1(+)-myc-SNAP-GP135Express myc and SNAP-tagged GP135 in mammmalian cells
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