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A genetically encoded tag for correlated light and electron microscopy of intact cells, tissues, and organisms.
Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, Ellisman MH, Tsien RY
PLoS Biol. 2011 Apr;9(4):e1001041. doi: 10.1371/journal.pbio.1001041. Epub 2011 Apr 5.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
54801mCherry-miniSOG-C1Localization: C1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528
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54802mCherry-miniSOG-Display-5Localization: Cytoplasm, Excitation: 448 / 473, Emission: 500 / 528
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54803mCherry-miniSOG-N1Localization: N1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528
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54804mCherry-miniSOG-Peroxisomes-2Localization: Peroxisomes, Excitation: 448 / 473, Emission: 500 / 528
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54821miniSOG-C1Localization: C1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528
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54822miniSOG-Display-5Localization: Cytoplasm, Excitation: 448 / 473, Emission: 500 / 528
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54823miniSOG-Lifeact-7Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528
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54824miniSOG-N1Localization: N1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528
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54846PA-mCherry-miniSOG-Lifeact-7Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528
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54847PA-mCherry-miniSOG-Peroxisomes-2Localization: Peroxisomes, Excitation: 448 / 473, Emission: 500 / 528
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54854tdminiSOG-C1Localization: C1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528
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54855tdminiSOG-N1Localization: N1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528
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55085mCherry-miniSOG-Actin-7Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528
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55086mCherry-miniSOG-C-Src-7Localization: Membrane/Vesicles, Excitation: 448 / 473, Emission: 500 / 528
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55087mCherry-miniSOG-CDC42-C-10Localization: Membrane, Excitation: 448 / 473, Emission: 500 / 528
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55088mCherry-miniSOG-Clathrin-15Localization: Clathrin Vesicles, Excitation: 448 / 473, Emission: 500 / 528
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55089mCherry-miniSOG-EB3-7Localization: MT End Binding Protein, Excitation: 448 / 473, Emission: 500 / 528
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55090mCherry-miniSOG-H2B-C-10Localization: Nucleus/Histones, Excitation: 448 / 473, Emission: 500 / 528
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55091mCherry-miniSOG-Keratin-17Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 448 / 473, Emission: 500 / 528
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55092mCherry-miniSOG-Paxillin-22Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528
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55093mCherry-miniSOG-Profilin-10Localization: Cytoskeleton, Excitation: 448 / 473, Emission: 500 / 528
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55094mCherry-miniSOG-Rab5a-7Localization: Ras GTPase, Excitation: 448 / 473, Emission: 500 / 528
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55095mCherry-miniSOG-RatLC3Localization: Microtubule-Binding Protein, Excitation: 448 / 473, Emission: 500 / 528
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55096mCherry-miniSOG-Talin-N-10Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528
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55097mCherry-miniSOG-Tubulin-6Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528
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55098mCherry-miniSOG-VASP-5Localization: Actin/Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528
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55099mCherry-miniSOG-Vimentin-7Localization: Intermediate Filaments, Excitation: 448 / 473, Emission: 500 / 528
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55100mCherry-miniSOG-Vinculin-23Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528
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55101mCherry-miniSOG-Zyxin-6Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528
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57761miniSOG-Actin-7Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528
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57762miniSOG-Alpha-Actinin-19Localization: Alpha Actinin, Excitation: 448 / 473, Emission: 500 / 528
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57763miniSOG-Alpha-V-Integrin-25Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528
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57764miniSOG-Clathrin-15Localization: Clathrin Vesicles, Excitation: 448 / 473, Emission: 500 / 528
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57765miniSOG-EB3-7Localization: MT End Binding Protein, Excitation: 448 / 473, Emission: 500 / 528
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57766miniSOG-ER-5Localization: Endoplasmic Reticulum, Excitation: 448 / 473, Emission: 500 / 528
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57768miniSOG-Golgi-7Localization: Golgi, Excitation: 448 / 473, Emission: 500 / 528
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57769miniSOG-H2B-C-10Localization: Nucleus/Histones, Excitation: 448 / 473, Emission: 500 / 528
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57770miniSOG-Keratin-17Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 448 / 473, Emission: 500 / 528
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57771miniSOG-LaminB1-10Localization: Nuclear Envelope, Excitation: 448 / 473, Emission: 500 / 528
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57772miniSOG-MAP4-C-10Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528
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57773miniSOG-Mito-7Localization: Mitochondria, Excitation: 448 / 473, Emission: 500 / 528
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57774miniSOG-Rab5a-7Localization: Ras GTPase, Excitation: 448 / 473, Emission: 500 / 528
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57775miniSOG-RatLC3Localization: Microtubule-Binding Protein, Excitation: 448 / 473, Emission: 500 / 528
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57776miniSOG-Talin-C-10Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528
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57777miniSOG-Tubulin-6Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528
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57778miniSOG-VASP-5Localization: Actin/Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528
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57779miniSOG-Vimentin-7Localization: Intermediate Filaments, Excitation: 448 / 473, Emission: 500 / 528
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57780miniSOG-Vinculin-23Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528
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57781miniSOG-Zyxin-6Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528
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57782PA-mCherry-miniSOG-Alpha-Actinin-19Localization: Alpha Actinin, Excitation: 448 / 473, Emission: 500 / 528
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57784PA-mCherry-miniSOG-CAF1-C-10Localization: Chromatin Assembly Factor 1, Excitation: 448 / 473, Emission: 500 / 528
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57785PA-mCherry-miniSOG-C-Src-7Localization: Membrane/Vesicles, Excitation: 448 / 473, Emission: 500 / 528
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57786PA-mCherry-miniSOG-Cx26-7Localization: Gap Junctions, Excitation: 448 / 473, Emission: 500 / 528
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57787PA-mCherry-miniSOG-Cx43-7Localization: Gap Junctions, Excitation: 448 / 473, Emission: 500 / 528
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57788PA-mCherry-miniSOG-EB3-7Localization: MT End Binding Protein, Excitation: 448 / 473, Emission: 500 / 528
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57789PA-mCherry-miniSOG-H2B-C-10Localization: Nucleus/Histones, Excitation: 448 / 473, Emission: 500 / 528
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57791PA-mCherry-miniSOG-Golgi-7Localization: Golgi, Excitation: 448 / 473, Emission: 500 / 528
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57792PA-mCherry-miniSOG-Keratin-17Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 448 / 473, Emission: 500 / 528
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57793PA-mCherry-miniSOG-Lysosomes-20Localization: Lysosome Membrane, Excitation: 448 / 473, Emission: 500 / 528
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57794PA-mCherry-miniSOG-MAP4-C-10Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528
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57795PA-mCherry-miniSOG-Mito-7Localization: Mitochondria, Excitation: 448 / 473, Emission: 500 / 528
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57796PA-mCherry-miniSOG-Paxillin-22Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528
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57797PA-mCherry-miniSOG-Talin-N-10Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528
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57799PA-mCherry-miniSOG-VE-Cadherin-N-10Localization: Tight Junctions, Excitation: 448 / 473, Emission: 500 / 528
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57800PA-mCherry-miniSOG-Vimentin-7Localization: Intermediate Filaments, Excitation: 448 / 473, Emission: 500 / 528
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57801PA-mCherry-miniSOG-Vinculin-23Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528
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57802PA-mCherry-miniSOG-Zyxin-6Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528
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57803tdminiSOG-Actin-7Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528
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57804tdminiSOG-H2B-C-10Localization: Nucleus/Histones, Excitation: 448 / 473, Emission: 500 / 528
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57805tdminiSOG-MAP4-C-10Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528
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57806tdminiSOG-PCNA-19Localization: Nucleus, Excitation: 448 / 473, Emission: 500 / 528
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57807tdminiSOG-RatLC3Localization: Microtubule-Binding Protein, Excitation: 448 / 473, Emission: 500 / 528
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57808tdminiSOG-Tubulin-6Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528
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91774pBAD miniSOGBacterial expression of a fluorescent protein, mini Singlet Oxygen Generator (miniSOG)
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